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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Recombinant human XRCC1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA5-12071 targets XRCC1 in IHC (P), IP, and WB applications and shows reactivity with Human samples.
The MA5-12071 immunogen is recombinant human XRCC1 protein.
X-Ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNA ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half. . In testis XRCC1 is expressed at high levels. Cells with a mutation of this gene exhibit decreased single strand break repair and reduced recombination repair. They show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cells deficient in base-excision repair reveal cancer hallmarks originating from adjustments to genetic instability.
MA5-12071 was used in western blot to test if base excision repair-deficient cells are a source of pre-cancerous cells
|Markkanen E,Fischer R,Ledentcova M,Kessler BM,Dianov GL||Nucleic acids research (43:3667)||2015|
complementing defective; repair in Chinese hamster; X-ray repair complementing defective repair in Chinese hamster cells 1; X-ray repair cross-complementing protein 1; X-ray-repair