|Tested species reactivity||Human, Rat|
|Published species reactivity||Rabbit, Human, Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant human XRCC1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13412 targets XRCC1 in IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.
The MA5-13412 immunogen is recombinant human XRCC1 protein.
X-Ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNA ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half. In testis XRCC1 is expressed at high levels. Cells with a mutation of this gene exhibit decreased single strand break repair and reduced recombination repair. They show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Interaction between DNA Polymerase ß and BRCA1.
MA5-13412 was used in western blot to study the functional significance of the DNA polymerase-beta interaction with BRCA1
|Masaoka A,Gassman NR,Horton JK,Kedar PS,Witt KL,Hobbs CA,Kissling GE,Tano K,Asagoshi K,Wilson SH||PloS one (8:null)||2016|
Base excision repair defects invoke hypersensitivity to PARP inhibition.
MA5-13412 was used in western blot to
|Horton JK,Stefanick DF,Prasad R,Gassman NR,Kedar PS,Wilson SH||Molecular cancer research : MCR (12:1128)||2014|
Preventing oxidation of cellular XRCC1 affects PARP-mediated DNA damage responses.
MA5-13412 was used in western blot to study the effect of cellular XRCC1 oxidation on PARP-mediated DNA damage responses
|Horton JK,Stefanick DF,Gassman NR,Williams JG,Gabel SA,Cuneo MJ,Prasad R,Kedar PS,Derose EF,Hou EW,London RE,Wilson SH||DNA repair (12:774)||2013|
Subcellular distribution of a fluorescence-labeled combi-molecule designed to block epidermal growth factor receptor tyrosine kinase and damage DNA with a green fluorescent species.
MA5-13412 was used in western blot to study the subcellular distribution of a mixed EGFR-DNA targeting molecule utilizing a green labelled DNA-damaging moiety and a blue-labelled EGFR inhibitor
|Todorova MI,Larroque AL,Dauphin-Pierre S,Fang YQ,Jean-Claude BJ||Molecular cancer therapeutics (9:869)||2010|
Chk2-dependent phosphorylation of XRCC1 in the DNA damage response promotes base excision repair.
MA5-13412 was used in western blot to study the role of Chk2-dependent XRCC1 phosphorylation in the DNA damage response
|Chou WC,Wang HC,Wong FH,Ding SL,Wu PE,Shieh SY,Shen CY||The EMBO journal (27:3140)||2008|
Tamoxifen accelerates proteasomal degradation of O6-methylguanine DNA methyltransferase in human cancer cells.
MA5-13412 was used in western blot to study the role of tamoxifen in increasing proteasomal degradation of MGMT in human cancer cells
|Kuo CC,Liu JF,Shiah HS,Ma LC,Chang JY||International journal of cancer (121:2293)||2007|
Human Xip1 (C2orf13) is a novel regulator of cellular responses to DNA strand breaks.
MA5-13412 was used in western blot to study the role of human Xip1 in regulating cellular responses to DNA strand breaks
|Bekker-Jensen S,Fugger K,Danielsen JR,Gromova I,Sehested M,Celis J,Bartek J,Lukas J,Mailand N||The Journal of biological chemistry (282:19638)||2007|
An intronic polymorphism associated with increased XRCC1 expression, reduced apoptosis and familial breast cancer.
MA5-13412 was used in western blot to investigate the relation between XRCC1 expression and apoptosis in breast cancer cell lines
|Bu D,Tomlinson G,Lewis CM,Zhang C,Kildebeck E,Euhus DM||Breast cancer research and treatment (99:257)||2006|
XRCC1 and DNA polymerase beta interaction contributes to cellular alkylating-agent resistance and single-strand break repair.
MA5-13412 was used in western blot to study the role of XRCC1 and DNA polymerase beta in cellular alkylating-agent resistance and single-strand break repair
|Wong HK,Wilson DM||Journal of cellular biochemistry (95:794)||2005|
Involvement of poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III in an alternative route for DNA double-strand breaks rejoining.
MA5-13412 was used in western blot to study an alternative pathway for the repair of DNA double-strand breaks that involves poly(ADP-ribose) polymerase-1 and a XRCC1-DNA ligase III complex
|Audebert M,Salles B,Calsou P||The Journal of biological chemistry (279:55117)||2004|
Oxidative DNA damage and repair in experimental atherosclerosis are reversed by dietary lipid lowering.
MA5-13412 was used in western blot to study the effects of dietary lipid levels on DNA damage and DNA repair pathways in experimentally induced atherosclerotic plaques
|Martinet W,Knaapen MW,De Meyer GR,Herman AG,Kockx MM||Circulation research (88:733)||2001|
Clinicopathological and functional significance of XRCC1 expression in ovarian cancer.
MA5-13412 was used in immunohistochemistry to study the function and predictive value of XRCC1 in ovarian cancer
|Abdel-Fatah T,Sultana R,Abbotts R,Hawkes C,Seedhouse C,Chan S,Madhusudan S||International journal of cancer (132:2778)||2013|
The prognostic significance of ERCC1, BRCA1, XRCC1, and betaIII-tubulin expression in patients with non-small cell lung cancer treated by platinum- and taxane-based neoadjuvant chemotherapy and surgical resection.
MA5-13412 was used in immunohistochemistry to investigate the usefulness of specific gene expression in non-small cell lung cancer treatment prognosis
|Kang CH,Jang BG,Kim DW,Chung DH,Kim YT,Jheon S,Sung SW,Kim JH||Lung cancer (Amsterdam, Netherlands) (68:478)||2010|
The Concept of Divergent Targeting through the Activation and Inhibition of Receptors as a Novel Chemotherapeutic Strategy: Signaling Responses to Strong DNA-Reactive Combinatorial Mimicries.
MA5-13412 was used in immunocytochemistry and western blot to study the concept of divergent targeting through the activation and inhibition of receptors as a novel chemotherapeutic strategy
|Watt HL,Rachid Z,Jean-Claude BJ||Journal of signal transduction (2012:null)||2012|
XRCC1 protects against the lethality of induced oxidative DNA damage in nondividing neural cells.
MA5-13412 was used in immunocytochemistry to study the role of XRCC1 in protecting against the lethality of induced oxidative DNA damage in nondividing neural cells
|Kulkarni A,McNeill DR,Gleichmann M,Mattson MP,Wilson DM||Nucleic acids research (36:5111)||2008|
Replication-dependent and -independent responses of RAD18 to DNA damage in human cells.
MA5-13412 was used in immunocytochemistry to study the role of RAD18 in the responses to DNA damage in human cells
|Nakajima S,Lan L,Kanno S,Usami N,Kobayashi K,Mori M,Shiomi T,Yasui A||The Journal of biological chemistry (281:34687)||2006|
In situ analysis of repair processes for oxidative DNA damage in mammalian cells.
MA5-13412 was used in immunocytochemistry to study the mammalian repair mechanism for oxidation-induced DNA damage
|Lan L,Nakajima S,Oohata Y,Takao M,Okano S,Masutani M,Wilson SH,Yasui A||Proceedings of the National Academy of Sciences of the United States of America (101:13738)||2004|