|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||His-tagged human XRCC3, over-expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody does not react with mouse samples.
The target sequence has 100% sequence homology with non-human primate and 93% sequence homology with sheep, rat, and bovine.
Suggested positive control: antigen standard for XRCC3 (transient overexpression lysate).
Five human RAD51 homologs have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologs interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. XRCC3 (x-ray cross complementing) was first identified as a human gene complementing the DNA damage sensitivity, chromosonal instability and impaired growth of the mutant hamster cell line irs1SF. This fact coupled with XRCC3 being a member of the RAD51 gene family have implied a role in the repair of DNA damage by homologous recombination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.