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Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Breast Carcinoma tissue using PA5-19677, anti-Yap1 antibody. Primary antibody was used at a concentration of 5 ug/ml and exposed for 8 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6 / 20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human YAP1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western Blot, this antibody has non-specific bands at 73 kDa.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
65 kDa Yes-associated protein; protein yorkie homolog; transcriptional coactivator YAP1; YAP, YAP2, YAP65, YKI, 65 kDa Yes-associated protein; Yes-associated protein 1; Yes-associated protein 1, 65kDa; yes-associated protein 2; yes-associated protein beta; yes-associated protein delta; yes-associated protein YAP65 homolog; yes-associated protein, 65 kDa; yorkie homolog
AI325207; COB1; YAP; YAP1; YAP2; YAP65; YKI; Yorkie