Western blot analysis of HeLa Whole Cell Lysate using PA5-19453, YB1 primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19453 staining of Jurkat Whole Cell Lysate at a dilution of 1 ug/ml (Lane 2). PA5-19453 staining of MCF7 Whole Cell Lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human YB1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:5000|
|Western Blot (WB)||1-1.4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western Blot, this antibody has non-specific bands at 50 kDa.
This antibody is predicted to react with Xenopus laevis based on sequence homology.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.