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Western blot analysis of HeLa Whole Cell Lysate using PA5-19453, YB1 primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19453 staining of Jurkat Whole Cell Lysate at a dilution of 1 ug/ml (Lane 2). PA5-19453 staining of MCF7 Whole Cell Lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human YB1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:5000|
|Western Blot (WB)||1-1.4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western Blot, this antibody has non-specific bands at 50 kDa.
This antibody is predicted to react with Xenopus laevis based on sequence homology.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BP-8, CSDA2, CSDB, DBPB, MDR-NF1, MGC104858, MGC110976, MGC117250, NSEP-1, NSEP1, YB-1, YB1, CBF-A; CBF-A; CCAAT-binding transcription factor 1 subunit A; CCAAT-binding transcription factor I subunit A; DNA binding protein B; DNA-binding protein B; EFI-A; enhancer factor 1 alpha; enhancer factor 1 subunit A; enhancer factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I; nuclease sensitive element binding protein 1; nuclease-sensitive element-binding protein 1; Y box binding protein 1; Y box transcription factor; Y-box binding protein 1; Y-box transcription factor; Y-box-binding protein 1; YB-1
1700102N10Rik; BP-8; Byb1; C79409; CBF-A; Cbfa; CSDA2; CSDB; DBPB; EF1A; EFI-A; MDR-NF1; Msy-1; MSY1; mYB-1a; NSEP-1; NSEP1; YB-1; YB1; YBX1