|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||GST-fusion protein of Human ZAP70 of amino acids 282-307 [RRIDTLNSDGYTPEPARITSPDKPRP]|
|Storage buffer||PBS, pH 7.2|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||Assay dependent|
|Immunofluorescence (IF)||0.5 µg x 10^6 cells in 100 µl|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||0.5-1 µg/ml|
|Flow Cytometry (Flow)||See 3 publications below|
MA1-21350 detects ZAP70 in Human and Mouse samples.
MA1-21350 has been successfully used in Immunofluorescence, Flow cytometry, ELISA, Immunoprecipitation and Western blot procedures. Postive control of Jurkat cell lysate suggested.
MA1-21350 immunogen corresponds to GST-fusion protein of Human ZAP70 of amino acids 282-307 [RRIDTLNSDGYTPEPARITSPDKPRP].
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.
Methodological comparison of two anti-ZAP-70 antibodies.
MA1-21350 was used in flow cytometry to discuss variance in the detection of ZAP-70 expression by clinical laboratories.
|Degheidy HA,Venzon DJ,Farooqui MZ,Abbasi F,Arthur DC,Wilson WH,Wiestner A,Stetler-Stevenson MA,Marti GE||Cytometry. Part B, Clinical cytometry (80:300)||2011|
Prospective study of clinical and biological prognostic factors at diagnosis in patients with early stage B-cell chronic lymphocytic leukemia.
MA1-21350 was used in flow cytometry to identify prognostic factors for chronic lymphocytic leukemia using a retrospective series.
|Oliveira AC,de la Banda E,Domingo-Domenech E,Encuentra M,Mercadal S,Domingo A,Alonso E,Espinet B,Grau J,De Sevilla AF,Gonzalez-Barca E||Leukemia & lymphoma (52:429)||2011|
Comparison of methods for determining zeta-chain associated protein - 70 (ZAP-70) expression in patients with B-cell chronic lymphocytic leukemia (B-CLL).
MA1-21350 was used in flow cytometry to investigate novel monoclonal antibodies to ZAP-70 to detect B-cell chronic lymphocytic leukemia
|Bojarska-Junak A,Giannopoulos K,Kowal M,Dmoszyńska A,Roliński J||Cytometry. Part B, Clinical cytometry (70:293)||2006|
70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; FLJ17670; FLJ17679; SRK; STD; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP-70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
ADMIO2; IMD48; mrtle; mur; SRK; STCD; STD; TZK; ZAP-70; ZAP70