Human peripheral blood from normal donors was stained using a combination of anti-human CD3 monoclonal antibody (clone: S4.1), for the cell surface antigens, and anti-human / mouse Zap-70 monoclonal antibody (clone: 1E7.2), for intracellular detection. The cells were fixed and / or permeabilized using Fix and Perm solution (cat.# GAS-003 / 004). The profiles represent lymphocyte gate.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||GST-fusion protein of Human ZAP70, amino acids 282-307|
|Storage buffer||PBS with sucrose, 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
Mutations in ZAP-70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP-70 expression also defines a subset of Chronic Lymphocytic Leukaemia (CLL) in patients with unmutated Ig gene and poor clinical course.
Analyte Specific Reagent
Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia.
MHZAP7004-4 was used in flow cytometry to discuss using flow cytometry to assess Zap-70 in chronic lymphocytic leukemia.
|Vroblova V,Smolej L,Krejsek J||Hematology (Amsterdam, Netherlands) (17:268)||2012|
Methodological comparison of two anti-ZAP-70 antibodies.
MHZAP7004-4 was used in flow cytometry to discuss variance in the detection of ZAP-70 expression by clinical laboratories.
|Degheidy HA,Venzon DJ,Farooqui MZ,Abbasi F,Arthur DC,Wilson WH,Wiestner A,Stetler-Stevenson MA,Marti GE||Cytometry. Part B, Clinical cytometry (80:300)||2011|