For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
ZBED1, also known as DREF, is the human homolog of the Drosophila protein DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation. RNA interference (RNAi) experiments targeting ZBED1 RNA resulted in the inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells, suggesting that ZBED1 also plays a role in cell proliferation in human cells. At least 22 ribosomal proteins (RPs) possess ZBED1 binding sites in their promoters; anti-ZBED1 RNAi also resulted in decreased RP gene expression. Immunoprecipitation analyses have shown that ZBED1 self-associates in vivo via a carboxyl-terminal hATC domain, and this self-association is required for nuclear localization and DNA binding.
Protein Aliases: Ac-like transposable element; BED-type zinc finger domain-containing protein 1; DNA replication-related element binding factor; DNA replication-related element-binding factor; dREF homolog; E3 SUMO-protein ligase ZBED1; Putative Ac-like transposable element; Zinc finger BED domain-containing protein 1; zinc finger, BED-type containing 1
Gene Aliases: ALTE; DREF; hDREF; KIAA0785; TRAMP; ZBED1
UniProt ID: (Human) O96006
Entrez Gene ID: (Human) 9189
Molecular Function: DNA metabolism protein