Western blot of ZCCHC11/TUT4 in different testis lysates from wildtype 8-9 week old C57BL/6 (wt) and knock-out (KO) mice was performed using 35ug protein in RIPA buffer. Lysates were probed with a ZCCHC11/TUT4 polyclonal antibody (Product# PA5-37994) at a dilution of 0.2ug/ml and incubated for 2 hours, followed by chemiluminescent detection.
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-QKSAEKQGDLTPSET, from the internal region of mouse Zcchc11|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.03-0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ZCCHC11 is an RNA uridyltransferase that uses UTP to add uridines to the 3-prime end of substrate RNA molecules .
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.