Immunofluorescent analysis of ZFP281 (red) in human embryonic stem cell H9 line grown on irradiated MEF-feeder layer. The cells were fixed with 4% paraformaldehyde at room temperature for 10 min and permeabilized with 0.25% Triton-X 100 for 5 min and blocked with the 10% BSA in PBS for 30 min at 37°C. Cells were stained with a ZFP281 polyclonal antibody (Product # PA5-21121) at a dilution of 1:200 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with a RRX-conjugated donkey anti-rabbit IgG secondary antibody at a dilution of 1:500 for 1 hour at room temperature. Nucleus DNA (blue) was stained with DAPI (Product # D1306).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 19 amino acid peptide near the carboxy terminus of human ZFP281.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.25-0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is A-20 cell lysate.
PA5-21121 can be used with blocking peptide PEP-1235.
ZFP281, also known as ZBP-99, is a member of a conserved family of transcription factors that interact with GC-rich promoters. It contains four Kruppel-like zinc fingers and is highly homologous to ZBP-89, another regulator of ornithine decarboxylase gene expression. ZFP281 also interacts with many other other transcription factors, such as Sp1 to repress vimentin gene transcription. ZFP281 has also been shown to control embryonic stem cell pluripotency by direct regulating specific target genes. ZFP281 phyically interacts with the transcription factos POU5F1, Sox2, and NANOG. Gene expression microarray anlysis indicated that some ZFP281 target genes were activated, while others were repressed upon knockdown of ZFP281 expression, showing that ZFP281 plays bifuctinal roles in regulating gene expression in stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.