Invitrogen

ZFP57 Monoclonal Antibody (GT865)

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ZFP57 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of ZFP57 was performed in Mock and transfected 293T cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ZFP57 Monoclonal Antibody (GT865) (Product # MA5-27869) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

ZFP57 Antibody in Western Blot (WB) — Western blot analysis of ZFP57 in non-transfected (-) and ZFP57 -transfected (+) 293T whole cell extracts using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with ZFP57 monoclonal antibody (Product # MA5-27869) using a dilution of 1:5000.

Product Details

MA5-27869

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:10,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	GT865
Immunogen	Recombinant protein encompassing a sequence within the N-terminus region of human ZFP57. The exact sequence is proprietary. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2735415

Product Specific Information

Positive Control: 3xFlag-human ZFP57-transfected 293T

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is a zinc finger protein containing a KRAB domain. Studies in mouse suggest that this protein may function as a transcriptional repressor. Mutations in this gene have been associated with transient neonatal diabetes mellitus type 1 (TNDM1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.