OriGene

ZNF217 Monoclonal Antibody (OTI3G5), TrueMAB™

View all (24) ZNF217 antibodies

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Questions & Answers

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ZNF217 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-ZNF217 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809028)(1:150).

ZNF217 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-ZNF217 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809028)(1:150).

ZNF217 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human Kidney tissue within the normal limits using anti-ZNF217 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809028)(1:150).

ZNF217 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human liver tissue within the normal limits using anti-ZNF217 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809028)(1:150).

ZNF217 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human lung tissue within the normal limits using anti-ZNF217 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809028)(1:150).

ZNF217 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF217 (RC224371, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF21. Positive lysates LY416585 (100 µg) and LC416585 (20 µg) can be purchased separately from OriGene.

Product Details

TA809028

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI3G5
Immunogen	Human recombinant protein fragment corresponding to amino acids 730-1048 of human ZNF217 produced in E.coli. View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Zinc finger protein 217

Gene Aliases: ZABC1; ZNF217

UniProt ID: (Human) O75362

Entrez Gene ID: (Human) 7764

Molecular Function: C2H2 zinc finger transcription factor

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated signal transduction neurogenesis negative regulation of transcription, DNA-templated

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding transcription regulatory region DNA binding metal ion binding