Invitrogen

ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 488

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

14 Published Figures

35 References

View all (32) ZO-1 antibodies

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ZO-1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of ZO-1 was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 488 at 5µg/mL in 0.1% BSA and incubated for 3 hours at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing junctional localization. Panel e shows the isotype control. The images were captured at 60X magnification.

ZO-1 Antibody — Figure 7 Induction of EMT after CPT treatment of SKOV3 cells A. RT-qPCR expression analysis of the indicated EMT marker genes after the indicated times of CPT treatment (sample size: n >= 3). B. Flow cytometry analysis of intracellular SMA after 0, 2 or 21 weeks of CPT treatment (sample size: n = 3). Values represent the fold change (FC) in the number of positive cells relative to small cells on day 0 (normalized to 1). Cells gated for small cells (forward and sideward scatter as untreated SKOV3 cells) and larger cells (forward and sideward scatter larger than for SKOV3 cells). C. Flow cytometry analysis of IL-1beta expression in SMA-expressing SKOV3-R cells gated as in panel B. D. Labeling of actin filaments with California Red-conjugated phalloidin (red) after different periods of CPT exposure. E. Additional staining of the tight junction (zonula occludens) protein constituent ZO-1 by indirect immunofluorescence (green) in untreated SKOV3 and SKOV3-R cells. Nuclei were stained with DAPI in panels D and E. F. RT-qPCR analysis of VIM expression in SKOV3-R cells (21w) and the same cells grown in the absence of CPT for 7 days. G. Pro-inflammatory and EMT marker gene expression after cyclic CPT treatment (1 day CPT, 21 days recovery, 4 cycles) compared to untreated cells (sample size: n = 3). {TM}

ZO-1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Figure 2 RTpi-MI, RTdi-MI and RTgutGC cell lines are composed of a heterogeneous population. ( A ) PCR amplicons of stem cell ( sox9 , hopx and lgr5 ), epithelial ( zo-1 , cldn3 and e-cad ) and connective tissue ( col1a1 and vim ), enterocyte ( iap ) and goblet cell ( muc1 ) genes in RTpi-MI, RTdi-MI and RTgutGC cell lines. ( B ) Representative immunofluorescence microphotographs showing zonula occludens (Zo-1, green, picture at the top) and collagen type 1 (Col1a1, green, picture at the bottom). DAPI (blue) was used to stain cell nuclei. Scale bar, 50 um. ( C ) Representative immunofluorescence microphotographs showing the in situ hybridization of ppib (red) in RTpi-MI (picture at the top), RTdi-MI (picture in the middle) and RTgutGC (picture at the bottom). DAPI (blue) was used to stain cell nuclei. Scale bar, 10 um. ( D ) Graphs reporting the percentage of ppib -expressing cells in RTpi-MI, RTdi-MI and RTgutGC cell lines. Data are reported as percentages (mean +- SD from 3 independent experiments) of ppib + cells on total cell count. Different superscript letters indicate statistical significance.

ZO-1 Antibody in Immunohistochemistry (IHC) — Figure 7 Quantification of ileal mucosal barrier dysfunction, n=4 in each group. HE staining and immunohistochemical staining of ZO-1, Occludin, TLR-9 and TLR-4 in ileal mucosal; The rest of the columns represent the expression levels of ZO-1, Occludin, TLR-9 and TLR-4 in ileal mucosal. Data were shown as mean +- SD. *P<0.05; **P < 0.01. p values are calculated from T-test.

ZO-1 Antibody in Western Blot (WB) — Published figure using ZO-1 monoclonal antibody (Product # 339188) in Western Blot

Product Details

339188

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	View 4 publications 4 publications
Immunohistochemistry (IHC)	-	View 10 publications 10 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 2 publications 2 publications
Immunohistochemistry (PFA fixed) (IHC (PFA))	-	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	5 µg/mL	View 17 publications 17 publications
ELISA (ELISA)	0.1-1.0 µg/mL (unconjugated)	-

Product Specifications
Species Reactivity	Dog, Human
Published species	Bovine, Dog, Human, Mouse, Rat, Zebrafish
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	ZO1-1A12
Immunogen	Human recombinant ZO-1 fusion protein encompassing amino acids 334-634 View antigen
Conjugate	Alexa Fluor™ 488 Alexa Fluor 555 Alexa Fluor 594 Alexa Fluor 647 FITC Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 4mg/mL BSA
Contains	0.1% sodium azide
Storage conditions	4° C, store in dark
RRID	AB_2532187

Product Specific Information

This monoclonal antibody detects ZO-1alpha+ and ZO-1alpha- isoforms. The antibody is specific for ZO-1 proteins; cross-reactivity with the related ZO-2 protein has not been observed. Species reactivity includes human (Caco-2 Cell line) and dog (MDCK cell line). Based on sequence homology, reactivity with other species is likely but has not been confirmed.

For Alexa Fluor 488 excitation 495 nm/emission 519 nm. Each lot of this antibody is tested for specificity by immunofluorescence. Expires one year from date of receipt when stored as instructed.

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cryopreservation of human cerebral microvascular endothelial cells and astrocytes in suspension and monolayers.

PloS one

Marquez-Curtis LA,Bokenfohr R,McGann LE,Elliott JAW

Published figure using ZO-1 monoclonal antibody (Product # 339188) in Immunocytochemistry

Thu Sep 23 00:00:00 EDT 2021

Three-Dimensional Aggregated Spheroid Model of Hepatocellular Carcinoma Using a 96-Pillar/Well Plate.

Molecules (Basel, Switzerland)

Lee SY,Teng Y,Son M,Ku B,Hwang HJ,Tergaonkar V,Chow PK,Lee DW,Nam DH

339188 was used in Western Blot to we proposed a 3D aggregated spheroid model (ASM) to create large single spheroids by aggregating cells in Matrigel attached to the surface of 96-pillar plates during cell culture.

Mon Aug 16 00:00:00 EDT 2021

The Role of Intestinal Dysbacteriosis Induced Arachidonic Acid Metabolism Disorder in Inflammaging in Atherosclerosis.

Frontiers in cellular and infection microbiology

Sun Y,Wu D,Zeng W,Chen Y,Guo M,Lu B,Li H,Sun C,Yang L,Jiang X,Gao Q

Published figure using ZO-1 monoclonal antibody (Product # 339188) in Immunohistochemistry

Fri Jul 02 00:00:00 EDT 2021

New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo.

Cells

Pasquariello R,Verdile N,Pavlovic R,Panseri S,Schirmer K,Brevini TAL,Gandolfi F

Published figure using ZO-1 monoclonal antibody (Product # 339188) in Immunocytochemistry

Sat Jun 19 00:00:00 EDT 2021

New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo.

Cells

Pasquariello R,Verdile N,Pavlovic R,Panseri S,Schirmer K,Brevini TAL,Gandolfi F

Published figure using ZO-1 monoclonal antibody (Product # 339188) in Immunocytochemistry

Sat Jun 19 00:00:00 EDT 2021

Start codon disruption with CRISPR/Cas9 prevents murine Fuchs' endothelial corneal dystrophy.

eLife

Uehara H,Zhang X,Pereira F,Narendran S,Choi S,Bhuvanagiri S,Liu J,Ravi Kumar S,Bohner A,Carroll L,Archer B,Zhang Y,Liu W,Gao G,Ambati J,Jun AS,Ambati BK

339188 was used in Immunohistochemistry (Paraffin) to demonstrate if CRISPR/Cas9-based postnatal gene editing achieves structural and functional rescue in a mouse model of fuchs' endothelial corneal dystrophy.

Tue Jun 08 00:00:00 EDT 2021

Loss of Ciliary Gene Bbs8 Results in Physiological Defects in the Retinal Pigment Epithelium.

Frontiers in cell and developmental biology

Schneider S,De Cegli R,Nagarajan J,Kretschmer V,Matthiessen PA,Intartaglia D,Hotaling N,Ueffing M,Boldt K,Conte I,May-Simera HL

339188 was used in Immunocytochemistry to highlights the benefit of combinatorial "omics" approaches with in vivo data for investigating the function of ciliopathy proteins and emphasizes the importance of ciliary proteins in the RPE and their contribution to visual disorders.

Wed Mar 10 00:00:00 EST 2021

Rapid Manufacturing of Multilayered Microfluidic Devices for Organ on a Chip Applications.

Sensors (Basel, Switzerland)

Paoli R,Di Giuseppe D,Badiola-Mateos M,Martinelli E,Lopez-Martinez MJ,Samitier J

339188 was used in Immunocytochemistry-immunoflourescence to explore DM and RP technologies for fabrication of an OoC with an embedded membrane after the evaluation of the main limitations of PDMS soft-lithography strategy.

Tue Feb 16 00:00:00 EST 2021

The molecular dynamics of subdistal appendages in multi-ciliated cells.

Nature communications

Ryu H,Lee H,Lee J,Noh H,Shin M,Kumar V,Hong S,Kim J,Park S

339188 was used in Immunohistochemistry (PFA fixed) to show FGFR1 Oncogene Partner (FOP) is a useful marker for delineating the transformation of a circular, unpolarized subdistal appendage into a polarized structure with a basal foot.

Wed Jan 27 00:00:00 EST 2021

Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes.

ACS nano

Huang X,Shi X,Hansen ME,Setiady I,Nemeth CL,Celli A,Huang B,Mauro T,Koval M,Desai TA

339188 was used in Immunocytochemistry to suggest that these cytosolic structures are important intermediates formed in response to nanotopographic cues that facilitate rapid tight junction remodeling in order to regulate paracellular permeability.

Tue Oct 27 00:00:00 EDT 2020

Maternal administration of probiotics promotes gut development in mouse offsprings.

PloS one

Yu Y,Lu J,Oliphant K,Gupta N,Claud K,Lu L

339188 was used in Immunohistochemistry to investigate if maternal administration of probiotics to pregnant mouse dams can recapitulate the beneficial effects observed in neonates fed with probiotics directly.

Thu Oct 08 00:00:00 EDT 2020

A Novel Three-Dimensional Glioma Blood-Brain Barrier Model for High-Throughput Testing of Tumoricidal Capability.

Frontiers in oncology

Sherman H,Rossi AE

339188 was used in Immunohistochemistry to demonstrate proof-of-concept of a three-dimensional model to study label-free BBB transport as well as the resulting brain tumor cytotoxicity by combining two commercially available products: Corning® HTS Transwell®-96 tissue culture system and Corning 96-well spheroid microplates.

Thu Oct 01 00:00:00 EDT 2020

Tbx20 Induction Promotes Zebrafish Heart Regeneration by Inducing Cardiomyocyte Dedifferentiation and Endocardial Expansion.

Frontiers in cell and developmental biology

Fang Y,Lai KS,She P,Sun J,Tao W,Zhong TP

339188 was used in Immunohistochemistry-immunofluorescence to demonstrate that Tbx20 induction promotes adult heart regeneration by inducing cardiomyocyte dedifferentiation as well as non-cell-autonomously enhancing endocardial cell regeneration.

Mon Sep 28 00:00:00 EDT 2020

The impact of synthetic amorphous silica (E 551) on differentiated Caco-2 cells, a model for the human intestinal epithelium.

Toxicology in vitro : an international journal published in association with BIBRA

Hempt C,Kaiser JP,Scholder O,Buerki-Thurnherr T,Hofmann H,Rippl A,Schuster TB,Wick P,Hirsch C

339188 was used in Immunocytochemistry to investigate if food-relevant synthetic amorphous silica elicit acute toxicity responses in intestinal epithelial cells.

Tue Sep 01 00:00:00 EDT 2020

Adhesion of Epithelial Cells to PNIPAm Treated Surfaces for Temperature-Controlled Cell-Sheet Harvesting.

ACS applied materials & interfaces

Kim H,Witt H,Oswald TA,Tarantola M

339188 was used in Immunocytochemistry-immunoflourescence to show that these two ECM components differ in their detachment properties from PNIPAm microgel films upon cell harvesting, and thus gained a deeper understanding of cell-sheet maturation and harvesting process and the involved partial ECM dissolution.

Wed Jul 29 00:00:00 EDT 2020

Magnesium and vitamin C supplementation attenuates steroid-associated osteonecrosis in a rat model.

Biomaterials

Zheng LZ,Wang JL,Xu JK,Zhang XT,Liu BY,Huang L,Zhang R,Zu HY,He X,Mi J,Pang QQ,Wang XL,Ruan YC,Zhao DW,Qin L

339188 was used in Immunocytochemistry-immunoflourescence to investigate the effect of magnesium (Mg) and vitamin C (VC) supplementation for preventing steroid-associated osteonecrosis (SAON) in a rat experimental model.

Wed Apr 01 00:00:00 EDT 2020

Regional Differences in Tight Junction Protein Expression in the Blood-DRG Barrier and Their Alterations after Nerve Traumatic Injury in Rats.

International journal of molecular sciences

Lux TJ,Hu X,Ben-Kraiem A,Blum R,Chen JT,Rittner HL

Published figure using ZO-1 monoclonal antibody (Product # 339188) in Immunofluorescence

Tue Dec 31 00:00:00 EST 2019

Human intestinal spheroids cultured using Sacrificial Micromolding as a model system for studying drug transport.

Scientific reports

Samy KE,Levy ES,Phong K,Demaree B,Abate AR,Desai TA

339188 was used in Immunohistochemistry to report that geometrically controlled self-assembling multicellular intestinal Caco-2 spheroids cultured using Sacrificial Micromolding display reproducible intestinal features and functions that are more representative of the in vivo small intestine than the widely used 2D transwell model.

Tue Jul 09 00:00:00 EDT 2019

PI3K-Yap activity drives cortical gyrification and hydrocephalus in mice.

eLife

Roy A,Murphy RM,Deng M,MacDonald JW,Bammler TK,Aldinger KA,Glass IA,Millen KJ

339188 was used in Immunohistochemistry to study the mechanisms driving the initiation of brain folding.

Thu May 16 00:00:00 EDT 2019

Human gut derived-organoids provide model to study gluten response and effects of microbiota-derived molecules in celiac disease.

Scientific reports

Freire R,Ingano L,Serena G,Cetinbas M,Anselmo A,Sapone A,Sadreyev RI,Fasano A,Senger S

339188 was used in Immunocytochemistry-immunoflourescence to validate the use of patient-derived organoids monolayers as a novel tool for the study of CD.

Tue May 07 00:00:00 EDT 2019

Generation of an inducible RPE-specific Cre transgenic-mouse line.

PloS one

Schneider S,Hotaling N,Campos M,Patnaik SR,Bharti K,May-Simera HL

339188 was used in Immunohistochemistry-immunofluorescence to study how retinal pigment epithelium dysregulation affects visual function.

Mon Apr 22 00:00:00 EDT 2019

Long-Term In Vitro Expansion of Epithelial Stem Cells Enabled by Pharmacological Inhibition of PAK1-ROCK-Myosin II and TGF-β Signaling.

Cell reports

Zhang C,Lee HJ,Shrivastava A,Wang R,McQuiston TJ,Challberg SS,Pollok BA,Wang T

339188 was used in Immunocytochemistry-immunoflourescence to develop a method for one trillion-fold expansion of human epithelial stem and progenitor cells from skin, airway, mammary, and prostate glands in the absence of feeder cells.

Tue Oct 16 00:00:00 EDT 2018

EPHB6 augments both development and drug sensitivity of triple-negative breast cancer tumours.

Oncogene

Toosi BM,El Zawily A,Truitt L,Shannon M,Allonby O,Babu M,DeCoteau J,Mousseau D,Ali M,Freywald T,Gall A,Vizeacoumar FS,Kirzinger MW,Geyer CR,Anderson DH,Kim T,Welm AL,Siegel P,Vizeacoumar FJ,Kusalik A,Freywald A

Published figure using ZO-1 monoclonal antibody (Product # 339188) in Immunofluorescence

Sun Jul 01 00:00:00 EDT 2018

SNX16 Regulates the Recycling of E-Cadherin through a Unique Mechanism of Coordinated Membrane and Cargo Binding.

Structure (London, England : 1993)

Xu J,Zhang L,Ye Y,Shan Y,Wan C,Wang J,Pei D,Shu X,Liu J

339188 was used in Immunocytochemistry-Immunofluorescence to study the mechanism by which SNX16 regulates the recycling trafficking of E-cadherin.

Tue Aug 01 00:00:00 EDT 2017

A multi-stage process including transient polyploidization and EMT precedes the emergence of chemoresistent ovarian carcinoma cells with a dedifferentiated and pro-inflammatory secretory phenotype.

Oncotarget

Rohnalter V,Roth K,Finkernagel F,Adhikary T,Obert J,Dorzweiler K,Bensberg M,Müller-Brüsselbach S,Müller R

Published figure using ZO-1 monoclonal antibody (Product # 339188) in Immunofluorescence

Tue Nov 24 00:00:00 EST 2015

Cytoskeleton remodelling of confluent epithelial cells cultured on porous substrates.

Journal of the Royal Society, Interface

Rother J,Büchsenschütz-Göbeler M,Nöding H,Steltenkamp S,Samwer K,Janshoff A

339188 was used in immunocytochemistry to investigate the effect of substrate topography on cytoskeleton of epithelial cells

Fri Feb 06 00:00:00 EST 2015

rpS6 regulates blood-testis barrier dynamics through Akt-mediated effects on MMP-9.

Journal of cell science

Mok KW,Mruk DD,Cheng CY

Published figure using ZO-1 monoclonal antibody (Product # 339188) in Immunofluorescence

Sat Nov 15 00:00:00 EST 2014

rpS6 regulates blood-testis barrier dynamics through Akt-mediated effects on MMP-9.

Journal of cell science

Mok KW,Mruk DD,Cheng CY

Published figure using ZO-1 monoclonal antibody (Product # 339188) in Immunofluorescence

Sat Nov 15 00:00:00 EST 2014

rpS6 regulates blood-testis barrier dynamics through Akt-mediated effects on MMP-9.

Journal of cell science

Mok KW,Mruk DD,Cheng CY

Published figure using ZO-1 monoclonal antibody (Product # 339188) in Immunofluorescence

Sat Nov 15 00:00:00 EST 2014

Thrombin induces epithelial-mesenchymal transition and collagen production by retinal pigment epithelial cells via autocrine PDGF-receptor signaling.

Investigative ophthalmology & visual science

Bastiaans J,van Meurs JC,van Holten-Neelen C,Nagtzaam NM,van Hagen PM,Chambers RC,Hooijkaas H,Dik WA

339188 was used in immunocytochemistry to investigate the effect of the coagulation proteins factor Xa and thrombin on the epithelial-mesenchymal transition and collagen production by RPE cells.

Thu Dec 19 00:00:00 EST 2013

Primordium of an artificial Bruch's membrane made of nanofibers for engineering of retinal pigment epithelium cell monolayers.

Acta biomaterialia

Warnke PH,Alamein M,Skabo S,Stephens S,Bourke R,Heiner P,Liu Q

339188 was used in immunocytochemistry to test if nanofibrous membranes imitate the natural Bruch's membrane and allow for the engineering of an in vivo-like human retinal pigment epithelium.

Sun Dec 01 00:00:00 EST 2013

NLRP3 inflammasome blockade inhibits VEGF-A-induced age-related macular degeneration.

Cell reports

Marneros AG

Published figure using ZO-1 monoclonal antibody (Product # 339188) in Immunofluorescence

Thu Sep 12 00:00:00 EDT 2013

Renal intercalated cells are rather energized by a proton than a sodium pump.

Proceedings of the National Academy of Sciences of the United States of America

Chambrey R,Kurth I,Peti-Peterdi J,Houillier P,Purkerson JM,Leviel F,Hentschke M,Zdebik AA,Schwartz GJ,Hübner CA,Eladari D

Published figure using ZO-1 monoclonal antibody (Product # 339188) in Immunofluorescence

Tue May 07 00:00:00 EDT 2013

rpS6 Regulates blood-testis barrier dynamics by affecting F-actin organization and protein recruitment.

Endocrinology

Mok KW,Mruk DD,Silvestrini B,Cheng CY

339188 was used in Western Blotting to demonstrate that ribosomal protein S6 is a major regulator of F-actin organisation and adhesion protein recruitment at the blood-testis barrier.

Mon Oct 01 00:00:00 EDT 2012

Microtubule affinity-regulating kinase 4 (MARK4) is a component of the ectoplasmic specialization in the rat testis.

Spermatogenesis

Tang EI,Xiao X,Mruk DD,Qian XJ,Mok KW,Jenardhanan P,Lee WM,Mathur PP,Cheng CY

339188 was used in immunohistochemistry - frozen section to examine the role of actin and tubulin in ectoplasmic specialization

Sun Apr 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

Gene Aliases: TJP1; ZO-1; ZO1

UniProt ID: (Dog) O97758, (Human) Q07157

Entrez Gene ID: (Dog) 403752, (Human) 7082

Molecular Function: tight junction