Invitrogen

ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 594

6 Published Figures

15 References

View all (28) ZO-1 antibodies

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Datasheet

Questions & Answers

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ZO-1 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of ZO-1/TJP1 Antibody, Alexa Fluor® 594 conjugate (ZO1-1A12) was done on 90% confluent log phase CaCo2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO-1/TJP1 Antibody, Alexa Fluor® 594 conjugate (ZO1-1A12) (Product # 339194) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature (Panel a: red). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c: red). Panel c is a merged image showing cell junctional localization. Panel d is a no primary antibody control. The images were captured at 40X magnification.

ZO-1 Antibody — Figure 6 Detection of differentiated cells in NHBE pseudostratified epithelial layer generated from passage 1 cultures. Immunofluorescence for ( A ) Ciliated cells (alpha-tubulin, green), ( B ) goblet cells (MUC5B, green), ( C ) goblet cells (MUC5AC, red), ( D ) ZO-1 tight junctions (red), nuclear stain (DAPI - blue); and staining for ( E ) periodic acid-Schiff/alcian blue for mucus producing goblet cells.

Product Details

Applications

Tested Dilution

Publications

ELISA (ELISA)

0.1-1.0 µg/mL

Immunocytochemistry (ICC)

1-10 µg/mL

View 4 publications 4 publications

Immunofluorescence (IF)

1-10 µg/mL

View 7 publications 7 publications

Western Blot (WB)

1-2 µg/mL

Immunohistochemistry (IHC)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human

Published species

Chicken, Human, Mouse

Host / Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ZO1-1A12

Immunogen

Human recombinant ZO-1 fusion protein encompassing amino acids 334-634

Conjugate

Alexa Fluor® 594

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 4.0mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

RRID

AB_2532188

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

Gene Aliases: TJP1; ZO-1; ZO1

UniProt ID: (Human) Q07157

Entrez Gene ID: (Dog) 403752, (Human) 7082

Molecular Function: cell junction protein tight junction