Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Immunocytochemistry (ICC/IF) |
1-10 µg/mL | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Dog, Human |
Published species |
Human |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
ZO1-1A12 |
Immunogen |
Human recombinant ZO-1 fusion protein encompassing amino acids 334-634 |
Conjugate |
Alexa Fluor™ 555
|
Excitation/Emission Profile |
View spectra |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.4, with 1mg/mL BSA |
Contains |
0.05% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_2663168 |
ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
Gene Aliases: TJP1; ZO-1; ZO1
UniProt ID: (Dog) O97758, (Human) Q07157
Entrez Gene ID: (Dog) 403752, (Human) 7082
Molecular Function:
tight junction
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