Invitrogen

ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 555

Product Image Gallery

ZO-1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ZO-1 (orange) in Caco-2 cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ZO-1 Monoclonal Antibody, AlexaFluor 555 conjugate (Product # MA3-39100-A555) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Product Details

MA3-39100-A555

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	1-10 µg/mL	View 1 publication 1 publication

Product Specifications
Species Reactivity	Dog, Human
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	ZO1-1A12
Immunogen	Human recombinant ZO-1 fusion protein encompassing amino acids 334-634 View antigen
Conjugate	Alexa Fluor™ 555 Alexa Fluor 488 Alexa Fluor 594 Alexa Fluor 647 FITC Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2663168

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Single-cell-resolved differentiation of human induced pluripotent stem cells into pancreatic duct-like organoids on a microwell chip.

Nature biomedical engineering

Wiedenmann S,Breunig M,Merkle J,von Toerne C,Georgiev T,Moussus M,Schulte L,Seufferlein T,Sterr M,Lickert H,Weissinger SE,Möller P,Hauck SM,Hohwieler M,Kleger A,Meier M

MA3-39100-A555 was used in Immunocytochemistry to eport the single-cell characterization of the differentiation of pancreatic duct-like organoids (PDLOs) from human induced pluripotent stem cells (hiPSCs) on a microwell chip that facilitates the uniform aggregation and chemical induction of hiPSC-derived pancreatic progenitors.

Sun Aug 01 00:00:00 EDT 2021

Single-cell-resolved differentiation of human induced pluripotent stem cells into pancreatic duct-like organoids on a microwell chip.

Nature biomedical engineering

Wiedenmann S,Breunig M,Merkle J,von Toerne C,Georgiev T,Moussus M,Schulte L,Seufferlein T,Sterr M,Lickert H,Weissinger SE,Möller P,Hauck SM,Hohwieler M,Kleger A,Meier M

Sun Aug 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

Gene Aliases: TJP1; ZO-1; ZO1

UniProt ID: (Dog) O97758, (Human) Q07157

Entrez Gene ID: (Dog) 403752, (Human) 7082

Molecular Function: tight junction