Invitrogen

ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 555

Product Image Gallery

ZO-1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ZO-1 (orange) in Caco-2 cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ZO-1 Monoclonal Antibody, AlexaFluor 555 conjugate (Product # MA3-39100-A555) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1-10 µg/mL

Immunofluorescence (IF)

1-10 µg/mL

Product Specifications

Species Reactivity

Dog, Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

ZO1-1A12

Immunogen

Human recombinant ZO-1 fusion protein encompassing amino acids 334-634

Conjugate

Alexa Fluor® 555

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2663168

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; ZO-1; ZO1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

Gene Aliases: TJP1; ZO-1; ZO1

UniProt ID: (Dog) O97758, (Human) Q07157

Entrez Gene ID: (Dog) 403752, (Human) 7082

Molecular Function: cell junction protein tight junction