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Immunofluorescent analysis of ZO-1 (green) in Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 30 minutes at room temperature. Cells were stained with or without ZO-1 rabbit polyclonal antibody (Product # 70-2300), at a concentration of 5ug/ml for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:1000 for 1 hour at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
|Tested species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Dog, Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminal region of human, dog, mouse, and rat ZO-1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 4 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunofluorescence (IF)||See 2 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
40-2300 has been successfully used in Immunofluorescence and Immunohistochemistry.
Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane- associated guanylate kinase (MAGUK) family and include occludin, ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An in vitro blood-brain barrier model combining shear stress and endothelial cell/astrocyte co-culture.
40-2300 was used in immunocytochemistry to evaluate an in vitro blood-brain barrier model.
|Takeshita Y,Obermeier B,Cotleur A,Sano Y,Kanda T,Ransohoff RM||Journal of neuroscience methods (232:165)||2014|
Echovirus 1 entry into polarized Caco-2 cells depends on dynamin, cholesterol, and cellular factors associated with macropinocytosis.
40-2300 was used in immunocytochemistry to investigate the contribution of macropinocytosis to echovirus cell entry.
|Krieger SE,Kim C,Zhang L,Marjomaki V,Bergelson JM||Journal of virology (87:8884)||2013|
Heme modulates intestinal epithelial cell activation: involvement of NADPHox-derived ROS signaling.
40-2300 was used in immunocytochemistry to study the effects of heme upon a nontransformed small intestine epithelial cell line.
|Barcellos-de-Souza P,Moraes JA,de-Freitas-Junior JC,Morgado-Díaz JA,Barja-Fidalgo C,Arruda MA||American journal of physiology. Cell physiology (304:C170)||2013|
The LKB1 tumor suppressor controls spindle orientation and localization of activated AMPK in mitotic epithelial cells.
40-2300 was used in immunocytochemistry to report that a germline LKB mutation in mice impairs spindle orientation in cells of the upper gastrointestinal tract and causes dramatic mislocalization of AMPK in mitotic cells.
|Wei C,Bhattaram VK,Igwe JC,Fleming E,Tirnauer JS||PloS one (7:null)||2012|
Impaired intestinal mucosal barrier upon ischemia-reperfusion: "patching holes in the shield with a simple surgical method".
40-2300 was used in immunohistochemistry - paraffin section to study the effect of ischemia-reperfusion on intestinal mucosal barrier
|Rosero O,Ónody P,Kovács T,Molnár D,Lotz G,Tóth S,Turóczi Z,Fülöp A,Garbaisz D,Harsányi L,Szijártó A||BioMed research international (2014:null)||2014|
|Dog||Not Cited||Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis.||Rosenbaek LL,Kortenoeven ML,Aroankins TS,Fenton RA||The Journal of biological chemistry (289:13347)||2014|
Luminal mitosis drives epithelial cell dispersal within the branching ureteric bud.
40-2300 was used in immunohistochemistry to observe ureteric bud cell behaviors in developing mouse kidneys.
|Packard A,Georgas K,Michos O,Riccio P,Cebrian C,Combes AN,Ju A,Ferrer-Vaquer A,Hadjantonakis AK,Zong H,Little MH,Costantini F||Developmental cell (27:319)||2013|
MUC4 overexpression augments cell migration and metastasis through EGFR family proteins in triple negative breast cancer cells.
40-2300 was used in immunohistochemistry to investigate the functional role of MUC4 in breast cancers.
|Mukhopadhyay P,Lakshmanan I,Ponnusamy MP,Chakraborty S,Jain M,Pai P,Smith LM,Lele SM,Batra SK||PloS one (8:null)||2013|
Evidence for phenotypic plasticity in aggressive triple-negative breast cancer: human biology is recapitulated by a novel model system.
40-2300 was used in western blot to use a novel cell-based model system to study phenotypic plasticity in triple-negative breast cancer.
|D'Amato NC,Ostrander JH,Bowie ML,Sistrunk C,Borowsky A,Cardiff RD,Bell K,Young LJ,Simin K,Bachelder RE,Delrow J,Dawson A,Yee LD,Mrózek K,Clay TM,Osada T,Seewaldt VL||PloS one (7:null)||2012|
|The canonical NF-kappaB pathway governs mammary tumorigenesis in transgenic mice and tumor stem cell expansion.||Liu M,Sakamaki T,Casimiro MC,Willmarth NE,Quong AA,Ju X,Ojeifo J,Jiao X,Yeow WS,Katiyar S,Shirley LA,Joyce D,Lisanti MP,Albanese C,Pestell RG||Cancer research (70:10464)||2010|
||Expression of zonula occludens-1 (ZO-1) and the transcription factor ZO-1-associated nucleic acid-binding protein (ZONAB)-MsY3 in glial cells and colocalization at oligodendrocyte and astrocyte gap junctions in mouse brain.||Penes MC,Li X,Nagy JI||The European journal of neuroscience (22:404)||2005|
|Mouse||Not Cited||Expression of zonula occludens-1 (ZO-1) and the transcription factor ZO-1-associated nucleic acid-binding protein (ZONAB)-MsY3 in glial cells and colocalization at oligodendrocyte and astrocyte gap junctions in mouse brain.||Penes MC,Li X,Nagy JI||The European journal of neuroscience (22:404)||2005|
tight junction protein 1 (zona occludens 1); tight junction protein ZO-1; ZO-1; zona occludens 1; zona occludens protein 1; zonula occludens 1 protein; zonula occludens protein 1
TJP1; ZO-1; ZO1