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|Tested species reactivity||Dog, Human|
|Published species reactivity||Dog, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant ZO-1 fusion protein encompassing amino acids 334-634|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS, pH 7.4, with 1% BSA, 50% glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||5-10 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 2 publications below|
Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane- associated guanylate kinase (MAGUK) family and include occludin, ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cytoskeleton remodelling of confluent epithelial cells cultured on porous substrates.
339188 was used in immunocytochemistry to investigate the effect of substrate topography on cytoskeleton of epithelial cells
|Rother J,Büchsenschütz-Göbeler M,Nöding H,Steltenkamp S,Samwer K,Janshoff A||Journal of the Royal Society, Interface / the Royal Society (12:null)||2015|
Primordium of an artificial Bruch's membrane made of nanofibers for engineering of retinal pigment epithelium cell monolayers.
339188 was used in immunocytochemistry to test if nanofibrous membranes imitate the natural Bruch's membrane and allow for the engineering of an in vivo-like human retinal pigment epithelium.
|Warnke PH,Alamein M,Skabo S,Stephens S,Bourke R,Heiner P,Liu Q||Acta biomaterialia (9:9414)||2013|
tight junction protein 1 (zona occludens 1); tight junction protein ZO-1; ZO-1; zona occludens 1; zona occludens protein 1; zonula occludens 1 protein; zonula occludens protein 1
TJP1; ZO-1; ZO1