Immunofluorescence analysis of ZO-1 / TJP1 Antibody, Alexa Fluor® 594 conjugate (ZO1-1A12) was done on 90% confluent log phase CaCo2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO-1 / TJP1 Antibody, Alexa Fluor® 594 conjugate (ZO1-1A12) (339194) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature (Panel a: red). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). Panel c: red). Panel c is a merged image showing cell junctional localization. Panel d is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Dog, Human|
|Published species reactivity||Mouse, Chicken|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant ZO-1 fusion protein encompassing amino acids 334-634|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||PBS, pH 7.4, with 4.0mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane- associated guanylate kinase (MAGUK) family and include occludin, ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Real-time estimation of paracellular permeability of cerebral endothelial cells by capacitance sensor array.
339194 was used in immunocytochemistry to evaluate capacitance sensor array for the estimation of paracellular permeability of cerebral endothelial cells
|Hyun Jo D,Lee R,Hyoung Kim J,Oh Jun H,Geol Lee T,Hun Kim J||Scientific reports (5:null)||2015|
Hox proteins drive cell segregation and non-autonomous apical remodelling during hindbrain segmentation.
339194 was used in immunohistochemistry (frozen) to study the role of vertebrate Hox proteins during the partitioning of the developing hindbrain into rhombomeres.
|Prin F,Serpente P,Itasaki N,Gould AP||Development (Cambridge, England) (141:1492)||2014|