|ELISA (ELISA)||0.1-1.0 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
|Immunocytochemistry (ICC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Dog, Human|
|Published species reactivity||Mouse Chicken|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Human recombinant ZO-1 fusion protein encompassing amino acids 334-634|
Alexa Fluor® 594
|Excitation/Emission Profile||View spectra|
|Storage buffer||PBS, pH 7.4, with 4.0mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage conditions||4° C, store in dark|
Tight junctions (or zonula occludens) are complexes of proteins that create intercellular boundaries selective, semi-permeable barriers in the paracellular pathway between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of a family of putative signal transduction membrane-associated guanylate kinase (MAGUK) proteins and include occludin, ZO-1, ZO-2, and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain (potential mediator of protein-protein interaction), an SH3 domain, and the guanylate kinase (GuK) domain found in many synapse-associated proteins. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a peripheral (nontransmembrane) membrane protein found on the cytoplasmic leaflet of tight junctions. ZO-1 has a MW of 225 kDa and is found in two isoforms that differ by the presence or absence of an 80-amino acid region known as 'motif-a' and constitutes a basis for classifying tight junctions. ZO-1 is homologous to the dlg (discs-large) protein of Drosophila and the C. elegans lin-2 gene product. Loss of dlg results in neoplastic overgrowth of imaginal discs and loss of epithelial apical/basal polarity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
Gene Aliases: TJP1; ZO-1; ZO1
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