Immunofluorescence analysis of ZO-2 / TJP2 Antibody (3E8D9) was done on 90% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO-2 / TJP2 Antibody (3E8D9)(374700) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cell junctional localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Dog, Human|
|Published species reactivity||Fish, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide derived from an internal amino acids of the human ZO-2 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Tight junction proteins (TJPs) belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. TJPs bind to the cytoplasmic C termini of junctional transmembrane proteins and link them to the actin cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
p38¿ mitogen-activated protein kinase regulates the expression of tight junction protein ZO-1 in differentiating human epidermal keratinocytes.
37-4700 was used in western blot to investigate the regulation of ZO-1 by p38delta mitogen-activated protein kinase in differentiating human epidermal keratinocytes
|Siljamäki E,Raiko L,Toriseva M,Nissinen L,Näreoja T,Peltonen J,Kähäri VM,Peltonen S||Archives of dermatological research (306:131)||2014|
Effect of cellular senescence on the albumin permeability of blood-derived endothelial cells.
37-4700 was used in immunocytochemistry and western blot to study permeability and tight junctions in endothelial cells derived from human cord blood.
|Cheung TM,Ganatra MP,Peters EB,Truskey GA||American journal of physiology. Heart and circulatory physiology (303:H1374)||2012|
|Fish||Not Cited||An immunochemical marker for goldfish Mauthner cells.||Flores CE,Ene S,Pereda AE||Journal of neuroscience methods (175:64)||2008|
|Not Applicable||Not Cited||
Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions.
37-4700 was used in flow cytometry to describe a cancer stem cell gene-expression signature
|Birnie R,Bryce SD,Roome C,Dussupt V,Droop A,Lang SH,Berry PA,Hyde CF,Lewis JL,Stower MJ,Maitland NJ,Collins AT||Genome biology (9:null)||2008|
|Not Applicable||Not Cited||
Cyclin D1 is transcriptionally down-regulated by ZO-2 via an E box and the transcription factor c-Myc.
37-4700 was used in immunoprecipitation to investigate the role of zona occludens-2 in the regulation of cyclin D1 transcription
|Huerta M,Muñoz R,Tapia R,Soto-Reyes E,Ramírez L,Recillas-Targa F,González-Mariscal L,López-Bayghen E||Molecular biology of the cell (18:4826)||2007|