Invitrogen

ZPR1 Monoclonal Antibody (LG1)

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Antibody Testing Data (3)
Published Figures (1)

ZPR1 Antibody in Immunocytochemistry (ICC/IF)

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ZPR1 Antibody (MA1-13003) in ICC/IF

Immunofluorescent analysis of ZPR1 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ZPR1 monoclonal antibody (Product # MA1-13003) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). ZPR1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

ZPR1 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

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Product Details

MA1-13003

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

LG1

Immunogen

murine ZPR1 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980362

Product Specific Information

MA1-13003 detects ZPR1 in human and mouse samples.

MA1-13003 has been successfully been used in Immunofluorescence and Western Blot applications, where a band of ~50 kDa is detected.

The immunogen used to produce MA1-13003 was mouse ZPR1 protein.

Target Information

The protein encoded by this gene is found in the cytoplasm of quiescent cells but translocates to the nucleolus in proliferating cells. The encoded protein interacts with survival motor neuron protein (SMN1) to enhance pre-mRNA splicing and to induce neuronal differentiation and axonal growth. Defects in this gene or the SMN1 gene can cause spinal muscular atrophy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Single Amino Acid Residue Regulates PTEN-Binding and Stability of the Spinal Muscular Atrophy Protein SMN.

Cells

Rademacher S,Detering NT,Schüning T,Lindner R,Santonicola P,Wefel IM,Dehus J,Walter LM,Brinkmann H,Niewienda A,Janek K,Varela MA,Bowerman M,Di Schiavi E,Claus P

MA1-13003 was used in Immunocytochemistry, Immunoprecipitation, and Western Blotting to identify regulatory phosphorylation sites that modulate function and stability.

Tue Nov 03 00:00:00 EST 2020

A Single Amino Acid Residue Regulates PTEN-Binding and Stability of the Spinal Muscular Atrophy Protein SMN.

Cells

Rademacher S,Detering NT,Schüning T,Lindner R,Santonicola P,Wefel IM,Dehus J,Walter LM,Brinkmann H,Niewienda A,Janek K,Varela MA,Bowerman M,Di Schiavi E,Claus P

MA1-13003 was used in Immunocytochemistry, Immunoprecipitation, and Western Blotting to identify regulatory phosphorylation sites that modulate function and stability.

Tue Nov 03 00:00:00 EST 2020

A Single Amino Acid Residue Regulates PTEN-Binding and Stability of the Spinal Muscular Atrophy Protein SMN.

Cells

Rademacher S,Detering NT,Schüning T,Lindner R,Santonicola P,Wefel IM,Dehus J,Walter LM,Brinkmann H,Niewienda A,Janek K,Varela MA,Bowerman M,Di Schiavi E,Claus P

MA1-13003 was used in Immunocytochemistry, Immunoprecipitation, and Western Blotting to identify regulatory phosphorylation sites that modulate function and stability.

Tue Nov 03 00:00:00 EST 2020

A Single Amino Acid Residue Regulates PTEN-Binding and Stability of the Spinal Muscular Atrophy Protein SMN.

Cells

Rademacher S,Detering NT,Schüning T,Lindner R,Santonicola P,Wefel IM,Dehus J,Walter LM,Brinkmann H,Niewienda A,Janek K,Varela MA,Bowerman M,Di Schiavi E,Claus P

MA1-13003 was used in Immunocytochemistry, Immunoprecipitation, and Western Blotting to identify regulatory phosphorylation sites that modulate function and stability.

Tue Nov 03 00:00:00 EST 2020

A ZPR1 mutation is associated with a novel syndrome of growth restriction, distinct craniofacial features, alopecia, and hypoplastic kidneys.

Clinical genetics

Ito YA,Smith AC,Kernohan KD,Pena IA,Ahmed A,McDonell LM,Beaulieu C,Bulman DE,Smidt A,Sawyer SL,Dyment DA,Boycott KM,Clericuzio CL

MA1-13003 was used in Western Blotting to study the association of a homozygous missense mutation in ZPR1 with a rare and recognisable multisystem syndrome.

Mon Oct 01 00:00:00 EDT 2018

Deficiency of the zinc finger protein ZPR1 causes neurodegeneration.

Proceedings of the National Academy of Sciences of the United States of America

Doran B,Gherbesi N,Hendricks G,Flavell RA,Davis RJ,Gangwani L

MA113003 was used in immunocytochemistry to study the role of zinc finger protein ZPR1 in neurodegeneration

Tue May 09 00:00:00 EDT 2006

ZPR1 is essential for survival and is required for localization of the survival motor neurons (SMN) protein to Cajal bodies.

Molecular and cellular biology

Gangwani L,Flavell RA,Davis RJ

MA1-13003 was used in immunohistochemistry to investigate the role of ZPR1 for the motor neurons (SMN) survival and the localization of the survival motor neurons (SMN) protein to Cajal bodies

Fri Apr 01 00:00:00 EST 2005

Coupled in vitro import of U snRNPs and SMN, the spinal muscular atrophy protein.

Molecular cell

Narayanan U,Achsel T,Lührmann R,Matera AG

MA1-13003 was used in immunoprecipitation and western blot to study the coupled nuclear import of U snRNPs and the survival of motor neurons protein

Fri Oct 22 00:00:00 EDT 2004

Spinal muscular atrophy disrupts the interaction of ZPR1 with the SMN protein.

Nature cell biology

Gangwani L,Mikrut M,Theroux S,Sharma M,Davis RJ

MA113003 was used in immunocytochemistry and western blot to investigate the changes of ZPR1-SMN interaction in Spinal muscular atrophy

Sun Apr 01 00:00:00 EST 2001

Spinal muscular atrophy disrupts the interaction of ZPR1 with the SMN protein.

Nature cell biology

Gangwani L,Mikrut M,Theroux S,Sharma M,Davis RJ

MA113003 was used in immunocytochemistry and western blot to investigate the changes of ZPR1-SMN interaction in Spinal muscular atrophy

Sun Apr 01 00:00:00 EST 2001

Bioinformatics

Protein Aliases: Zinc finger protein 259; Zinc finger protein ZPR1

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Gene Aliases: AI303781; Zfp259; ZNF259; ZPR1

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UniProt ID: (Human) Q2TAA0, (Mouse) Q62384

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Entrez Gene ID: (Human) 8882, (Mouse) 22687

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Function(s)

protein binding zinc ion binding receptor tyrosine kinase binding translation initiation factor binding metal ion binding

Process(es)

microtubule cytoskeleton organization inner cell mass cell proliferation trophectodermal cell proliferation mRNA processing multicellular organism development RNA splicing positive regulation of gene expression spinal cord development cell differentiation Cajal body organization regulation of myelination positive regulation of RNA splicing DNA endoreduplication positive regulation of protein import into nucleus positive regulation of growth axon development cellular response to epidermal growth factor stimulus positive regulation of transcription involved in G1/S transition of mitotic cell cycle apoptotic process involved in development pre-mRNA catabolic process negative regulation of motor neuron apoptotic process signal transduction cell proliferation

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ZPR1 Monoclonal Antibody (LG1)

ZPR1 Antibody (MA1-13003) in ICC/IF

Product Details

MA1-13003

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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