MA1-21350 detects ZAP70 in Human and Mouse samples.
MA1-21350 has been successfully used in Immunofluorescence, Flow cytometry, ELISA, Immunoprecipitation and Western blot procedures. Postive control of Jurkat cell lysate suggested.
MA1-21350 immunogen corresponds to GST-fusion protein of Human ZAP70 of amino acids 282-307 [RRIDTLNSDGYTPEPARITSPDKPRP].
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; FLJ17670; FLJ17679; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; TZK; ZAP-70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
Gene Aliases: ADMIO2; IMD48; mrtle; mur; SRK; STCD; STD; TZK; ZAP-70; ZAP70