|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH at an N-terminal added cysteine, corresponding to residues 134-150 of human Zyxin.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25162 detects Zyxin from human, mouse, and rat samples.
PA1-25162 has been successfully used in Western blot, and Immunoflourescence procedures.
The PA1-25162 immunogen is a synthetic peptide, conjugated to KLH at an N-terminal added cysteine, corresponding to residues 134-150 of human Zyxin.
Zyxin binds alpha-actinin and the CRP proteins and is implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. It has been suggested that Zyxin is involved in gene transcription by its association with transcription factors in the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.