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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol, 1mg/ml BSA|
|Contains||1.5mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||10-20 µg/ml|
|Immunofluorescence (IF)||10-20 ug/ml|
|Immunoprecipitation (IP)||10-20 µg/ml|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26434 detects active Caspase 9 from human samples.
PA1-26434 has been successfully used in immunoprecipitation, immunocytochemistry and Western blot procedures.
Store at 4°C short term. For long term storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
APAF-3; APAF3; apoptotic protease activating factor 3; apoptotic protease MCH-6; cas9; caspase 9, apoptosis-related cysteine peptidase; CASPASE-9c; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; protein phosphatase 1, regulatory subunit 56; RP11-265F14.3
APAF-3; APAF3; CASP9; ICE-LAP6; MCH6; PPP1R56