|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol, 1mg/ml BSA|
|Contains||1.5mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||10-20 µg/ml|
|Immunofluorescence (IF)||10-20 ug/ml|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA1-26435 detects active Caspase 9 from rat samples.
PA1-26435 has been successfully used in IF, ICC, and Western blot procedures.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Examination of caspase-dependent apoptotic and necrotic changes in rat kidney exposed to different doses of permethrin.
PA1-26435 was used in immunohistochemistry to study the ability of permethrin to induce apoptotic and necrotic changes in rat kidney
|Guvenc D,Kabak Y,Atmaca E,Aksoy A,Guvenc T||Biotechnic and histochemistry : official publication of the Biological Stain Commission (88:76)||2013|