Immunofluorescent analysis of alpha-1 Adrenergic Receptor (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Alpha-1 Adrenergic Receptor polyclonal antibody (Product # PA1-047) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Fruit fly, Human, Mouse, Rat, Zebrafish|
|Published species reactivity||Rat, Cat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues K(339) F S R E K K A A K T(349) of the 3rd intracellular loop of human A1AR.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:400|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-047 detects alpha-1 adrenergic receptor (A1AR) from human, mouse, rat, zebra fish (Danio rerio) and Drosophila samples.
PA1-047 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa protein representing the A1AR from mouse kidney membrane preparations, mouse liver, HeLa cell lysates, zebra fish and drosophila samples. Immunofluorescence staining of A1AR in mouse kidney distal tubule cells with PA1-047 results in staining of the plasma membrane.
The PA1-047 immunogen is a synthetic peptide corresponding to residues K(339) F S R E K K A A K T(349) of the 3rd intracellular loop of human A1AR. This sequence is 100% conserved in all A1AR subtypes examined. PA1-047 immunizing peptide (Cat. # PEP-216) is available for use in neutralization and control experiments.
Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence.
A1AR subtypes are found in numerous tissues and have been shown to be involved in the regulation of blood pressure due to changes in vascular tone and cardiac output. Effects on uterine contraction, hepatic glucose metabolism, heat shock protein 70 (HSP70), proto-oncogene expression, and mitogenesis have been linked to A1AR activation. Norepinephrine can increase nitric oxide synthase (NOS) levels in the hypothalamus by activating alpha-1 AR which may indirectly suppress the release of luteinizing hormone (LH), the androgens, estrogen, and progesterone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Predator Stress-Induced CRF Release Causes Enduring Sensitization of Basolateral Amygdala Norepinephrine Systems that Promote PTSD-Like Startle Abnormalities.
PA1-047 was used in immunohistochemistry - free floating to characterize PTSD-like startle abnormalities by predator stress-induced CRF release causing an enduring sensitization of basolateral amygdala norepinephrine systems
|Rajbhandari AK,Baldo BA,Bakshi VP||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:14270)||2015|
Loss of motoneurons in the ventral compartment of the rat hypoglossal nucleus following early postnatal exposure to alcohol.
PA1-047 was used in immunohistochemistry to study motoneuron loss in the rat hypoglossal nucleus venral compartment in response to alcohol exposure in the early post-natal period
|Stettner GM,Kubin L,Volgin DV||Journal of chemical neuroanatomy (52:87)||2013|
Caffeine and an adenosine A(2A) receptor antagonist prevent memory impairment and synaptotoxicity in adult rats triggered by a convulsive episode in early life.
PA1-047 was used in immunohistochemistry to investigate the effect of kainate on the status of hippocampal adenosine receptors
|Cognato GP,Agostinho PM,Hockemeyer J,Müller CE,Souza DO,Cunha RA||Journal of neurochemistry (112:453)||2010|
Alpha 1-noradrenergic system role in increased motivation for cocaine intake in rats with prolonged access.
PA1-047 was used in immunohistochemistry to investigate the possible effect of neuroadaptation in the central noradrenergic system on increased cocaine self-administration in rats with extended access
|Wee S,Mandyam CD,Lekic DM,Koob GF||European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology (18:303)||2008|
Activation of subfornical organ neurons in rats through pre- and postsynaptic alpha-adrenoceptors.
PA1-047 was used in immunohistochemistry to suggest that activation of subfornical organ neurons in rats are activated postsynaptically
|Honda E,Ono K,Kataoka S,Inenaga K||American journal of physiology. Regulatory, integrative and comparative physiology (290:R1646)||2006|
N-(2-Chloroethyl)-N-ethyl-2-bromobenzylamine reduces intracellular calcium response to noradrenaline in rat visual cortex.
PA1-047 was used in immunohistochemistry to study the effect of the noradrenergic neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine on intracellular calcium responses to noradrenaline in the visual cortex
|Yamamoto M,Imamura K,Kobayashi M,Nakadate K,Yokoyama C,Watanabe Y,Yamamoto M,Negi A||Neuroscience (107:209)||2001|
Effects of monocular deprivation on the expression pattern of alpha-1 and beta-1 adrenergic receptors in the kitten visual cortex.
PA1-047 was used in immunohistochemistry to study the regulation of adrenergic receptors by sensory afferents in kitten brains
|Nakadate K,Imamura K,Watanabe Y||Neuroscience research (40:155)||2001|
Enhanced role of adenosine A(2A) receptors in the modulation of LTP in the rat hippocampus upon ageing.
PA1-047 was used in immunocytochemistry to investigate the role of adenosine A2A receptors in hippocampal synaptic plasticity in rats
|Costenla AR,Diógenes MJ,Canas PM,Rodrigues RJ,Nogueira C,Maroco J,Agostinho PM,Ribeiro JA,Cunha RA,de Mendonça A||The European journal of neuroscience (34:12)||2011|
Left ventricular dysfunction in murine models of heart failure and in failing human heart is associated with a selective decrease in the expression of caveolin-3.
PA1-047 was used in western blot to investigate the relationship between caveolin-3 expression and left ventricular dysfunction
|Feiner EC,Chung P,Jasmin JF,Zhang J,Whitaker-Menezes D,Myers V,Song J,Feldman EW,Funakoshi H,Degeorge BR,Yelamarty RV,Koch WJ,Lisanti MP,McTiernan CF,Cheung JY,Bristow MR,Chan TO,Feldman AM||Journal of cardiac failure (17:253)||2011|
Blockade of adenosine A(1) receptors prevents methylphenidate-induced impairment of object recognition task in adult mice.
PA1-047 was used in western blot to investigate the involvement of adenosine A1 receptor system in the cognitive enhancing effect of methylphenidate
|Mioranzza S,Costa MS,Botton PH,Ardais AP,Matte VL,Espinosa J,Souza DO,Porciúncula LO||Progress in neuro-psychopharmacology and biological psychiatry (35:169)||2011|
Adenosine receptor A1 regulates polymorphonuclear cell trafficking and microvascular permeability in lipopolysaccharide-induced lung injury.
PA1-047 was used in western blot to investigate the role of adenosine receptor A1 in lipopolysaccharide-induced lung injury
|Ngamsri KC,Wagner R,Vollmer I,Stark S,Reutershan J||Journal of immunology (Baltimore, Md. : 1950) (185:4374)||2010|
Biochemistry and pharmacology of epitope-tagged alpha(1)-adrenergic receptor subtypes.
PA1-047 was used in western blot to study the biochemistry and pharmacology of epitope-tagged alpha (1)-adrenergic receptor subtypes in HEK293 cells
|Vicentic A,Robeva A,Rogge G,Uberti M,Minneman KP||The Journal of pharmacology and experimental therapeutics (302:58)||2002|
Transcriptional regulation of alpha1-adrenoceptor gene in the rat liver during different phases of sepsis.
PA1-047 was used in western blot to study the alpha1 adrenoceptor protein expression in the rat liver during different phases of sepsis
|Dong LW,Yang J,Tong LJ,Tang C,Liu MS||Biochimica et biophysica acta (1453:207)||1999|
Adenosine deamination sustains dendritic cell activation in inflammation.
PA1-047 was used in flow cytometry to investigate the function of adenosine deaminase in dendritic inflammatory responses
|Desrosiers MD,Cembrola KM,Fakir MJ,Stephens LA,Jama FM,Shameli A,Mehal WZ,Santamaria P,Shi Y||Journal of immunology (Baltimore, Md. : 1950) (179:1884)||2007|