Western blot analysis of alpha 1b Adrenergic Receptor using 50µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Alpha 1b Adrenergic Receptor polyclonal antibody (Product # PA5-27120) at a dilution of 1:1000.
|Tested species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 347 and 440 of alpha 1b Adrenergic Receptor|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27120 targets alpha 1b Adrenergic Receptor in WB applications and shows reactivity with mouse samples.
The PA5-27120 immunogen is a synthetic peptide corresponding to a region within amino acids 347 and 440 of alpha 1b Adrenergic Receptor.
Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This gene comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region.
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