|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Alpha 2-Macroglobulin purified from human plasma|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunoprecipitation (IP)||1 µg|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
A suggested positive control for this product is human plasma.
Alpha 2-Macroglobulin (a2M), is a ~720-kDa homotetrameric glycoprotein composed of four identical ~180 kDa subunits. aAlpha 2M shares with other alpha-macroglobulins, like the complement components C3 and C4 and the pregnancy zone protein PZP, an extraordinary binding capacity for a variety of ligands. This allows the alpha-macroglobulins to serve as humoral defense barriers against foreign peptides in the plasma. Alpha 2M interacts and captures virtually any proteinase, often referred to as a panprotease inhibitor. In the brain of Alzheimer's disease (AD) patients, Alpha 2M also has been localized to diffuse amyloid plaques, supporting an important role for alpha 2M in AD etiopathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Heterogeneity in neutrophil microparticles reveals distinct proteome and functional properties.
LF-MA0139 was used in flow cytometry and western blot to study the proteome of neutrophil microparticles in order to elucidate their biological functions
|Dalli J,Montero-Melendez T,Norling LV,Yin X,Hinds C,Haskard D,Mayr M,Perretti M||Molecular and cellular proteomics : MCP (12:2205)||2013|