Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:3,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:100 | - |
Immunocytochemistry (ICC/IF) |
1:100-1:500 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthesized peptide derived from N-terminal of human Actin alpha-2/3. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.4, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2635255 |
The antibody detects endogenous levels of total alpha Actinin 2 + alpha Actinin 3 protein.
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Actinin alpha3; actinin, alpha 2; actinin, alpha 3; Actinin-alpha3; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin skeletal muscle isoform 3; Alpha-actinin-2; Alpha-actinin-3; alpha-Actinin3; alphaActinin-3; F-actin cross-linking protein
Gene Aliases: 1110008F24Rik; ACTN2; ACTN3; CMD1AA; CMH23
UniProt ID: (Human) P35609, (Human) Q08043, (Mouse) Q9JI91, (Mouse) O88990
Entrez Gene ID: (Human) 88, (Human) 89, (Mouse) 11472, (Rat) 291245, (Mouse) 11474, (Rat) 171009
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