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Invitrogen

alpha Actinin 2/3 Polyclonal Antibody

View all (4) alpha Actinin 2/3 antibodies
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Product Details

PA5-49802

Applications Tested Dilution Publications

Western Blot (WB)

 1:500-1:3,000 -

Immunohistochemistry (Paraffin) (IHC (P))

 1:50-1:100 -

Immunocytochemistry (ICC/IF)

 1:100-1:500 -
Product Specifications

Species Reactivity

 Human, Mouse, Rat

Host/Isotype

 Rabbit / IgG

Class

 Polyclonal

Type

 Antibody

Immunogen

Synthesized peptide derived from N-terminal of human Actin alpha-2/3.
View antigen

Conjugate

 Unconjugated

Form

 Liquid

Concentration

 1 mg/mL

Purification

 Antigen affinity chromatography

Storage buffer

 PBS, pH 7.4, with 50% glycerol

Contains

 0.02% sodium azide

Storage conditions

 -20°C

RRID

AB_2635255

Product Specific Information

The antibody detects endogenous levels of total alpha Actinin 2 + alpha Actinin 3 protein.

Target Information

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Actinin alpha3; actinin, alpha 2; actinin, alpha 3; Actinin-alpha3; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin skeletal muscle isoform 3; Alpha-actinin-2; Alpha-actinin-3; alpha-Actinin3; alphaActinin-3; F-actin cross-linking protein

Gene Aliases: 1110008F24Rik; ACTN2; ACTN3; CMD1AA; CMH23

UniProt ID: (Human) P35609, (Human) Q08043, (Mouse) Q9JI91, (Mouse) O88990

Entrez Gene ID: (Human) 88, (Human) 89, (Mouse) 11472, (Rat) 291245, (Mouse) 11474, (Rat) 171009

Function(s)
MAPK cascade platelet degranulation cell adhesion microspike assembly muscle filament sliding regulation of membrane potential regulation of apoptotic process negative regulation of potassium ion transport positive regulation of potassium ion transport positive regulation of GTPase activity sarcomere organization focal adhesion assembly protein homotetramerization actin filament uncapping cardiac muscle cell development phospholipase C-activating angiotensin-activated signaling pathway establishment of protein localization to plasma membrane negative regulation of potassium ion transmembrane transporter activity positive regulation of potassium ion transmembrane transporter activity regulation of nucleic acid-templated transcription negative regulation of protein localization to cell surface positive regulation of receptor activity positive regulation of endocytic recycling positive regulation of cation channel activity regulation of the force of skeletal muscle contraction skeletal muscle atrophy transition between fast and slow fiber response to denervation involved in regulation of muscle adaptation negative regulation of glycolytic process positive regulation of skeletal muscle tissue growth negative regulation of calcineurin-NFAT signaling cascade negative regulation of oxidative phosphorylation regulation of aerobic respiration positive regulation of glucose catabolic process to lactate via pyruvate muscle contraction
Process(es)
Ras guanyl-nucleotide exchange factor activity integrin binding calcium ion binding protein binding phosphatidylinositol-4,5-bisphosphate binding cytoskeletal protein binding structural constituent of muscle protein domain specific binding LIM domain binding thyroid hormone receptor coactivator activity titin binding identical protein binding protein homodimerization activity ion channel binding protein dimerization activity actin filament binding FATZ binding titin Z domain binding actin binding ligand-dependent nuclear receptor transcription coactivator activity protein binding, bridging metal ion binding

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