|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between amino acids 70-140 of the human Crystallin AB protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
This Crystallin AB antibody is useful for Western blot analysis, where a band is seen at ~20 kDa.
There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex.
CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes.Suggested positive control: human and mouse skeletal muscle lysate, human skeletal muscle protein, antigen standard for HSPB1 (transient overexpression lysate), mouse skeletal muscle protein.
Crystallin AB (CryAB) is a developmentally regulated small heat shock protein known to protect cells from stress damage. It may also play an important role in the Golgi reorganization during cell division. CryAB is known to be associated with a number of neurodegenerative pathologies such as Alzheimer disease amd multiple sclerosis. As well, it has been found to be expressed in breast tumors of patients with shorter survival, suggesting that it may be a useful marker in identifying aggressive breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease.
PA1-16950 was used in immunohistochemistry to study the composition of the protein aggregate hallmark of Alexander disease called rosenthal fibers
|Heaven MR,Flint D,Randall SM,Sosunov AA,Wilson L,Barnes S,Goldman JE,Muddiman DC,Brenner M||Journal of proteome research (15:2265)||2016|