Immunofluorescent analysis of alpha Catenin using Catenin alpha Monoclonal Antibody (1G5) (Product# MA1-2000 ) shows staining in A2058 Cells. Catenin alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Catenin alpha (Product# MA1-2000 ) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||90 kDa MBP/alpha catenin fusion protein purified on amylose column.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||10 µg/ml|
|Immunofluorescence (IF)||10 ug/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-2000 detects alpha catenin from human samples.
MA1-2000 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~102 kDa protein representing alpha catenin from scc-1 cell extract. In Western blot, this antibody did not cross-react with mouse samples. MA1-2000 can also be used in immunoprecipitation and immunofluorescence procedures.
The MA1-2000 immunogen is a 90 kDa MBP/alpha catenin fusion protein purified via an amylose column.
Alpha catenin is a member of the b-catenin family of cytosolic proteins. Alpha catenin interacts with N-cadherin and b-catenin on the cytosolic side of adherins junctions to mediate the interaction with the actin cytoskeleton. Alpha catenin is a homodimer in solution, but dissociates to form a 1:1 heterodimer with b-catenin in order to interact with cadherins. Mice that have a-catenin ablated in their surface epithelium demonstrate reduced hair growth, multinucleated cells, extensive proliferation and a general cancerous phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Integration of left-right Pitx2 transcription and Wnt signaling drives asymmetric gut morphogenesis via Daam2.
MA1-2000 was used in immunoprecipitation to study the role of Daam2 in the mechanism by which Pitx2 and Wnt participate in asymmetric gut morphogenesis
|Welsh IC,Thomsen M,Gludish DW,Alfonso-Parra C,Bai Y,Martin JF,Kurpios NA||Developmental cell (26:629)||2013|
Characterization of cadherin-24, a novel alternatively spliced type II cadherin.
MA1-2000 was used in western blot to investigate the functional and structural properties of cadherin 24 and its alternative splicing variant
|Katafiasz BJ,Nieman MT,Wheelock MJ,Johnson KR||The Journal of biological chemistry (278:27513)||2003|
Mechanism of extracellular domain-deleted dominant negative cadherins.
MA1-2000 was used in western blot to investigate the functions of extracellular domain-deleted dominant-negative cadherin in cells
|Nieman MT,Kim JB,Johnson KR,Wheelock MJ||Journal of cell science (112 ( Pt 10):1621)||1999|
Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.
MA1-2000 was used in western blot to investigate the importance of N-cadherin in cell-cell adhesion
|Islam S,Carey TE,Wolf GT,Wheelock MJ,Johnson KR||The Journal of cell biology (135:1643)||1996|
Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin.
MA1-2000 was used in western blot to investigate the interaction of plakoglobin with N-cadherin and alpha catenin
|Sacco PA,McGranahan TM,Wheelock MJ,Johnson KR||The Journal of biological chemistry (270:20201)||1995|