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Immunofluorescent analysis of alpha Catenin was performed using 90% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Catenin (alpha-CAT-7A4) Mouse Monoclonal Antibody (13-9700) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cell junctional localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Chicken, Human, Mouse, Rat, Clawed frog|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide derived from the C-terminus of mouse alpha-catenin.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/mL|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Alpha catenin is a member of the b-catenin family of cytosolic proteins. Alpha catenin interacts with N-cadherin and b-catenin on the cytosolic side of adherins junctions to mediate the interaction with the actin cytoskeleton. Alpha catenin is a homodimer in solution, but dissociates to form a 1:1 heterodimer with b-catenin in order to interact with cadherins. Mice that have a-catenin ablated in their surface epithelium demonstrate reduced hair growth, multinucleated cells, extensive proliferation and a general cancerous phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis.
13-9700 was used in immunohistochemistry to study the role of plastin 3 in ectoplasmic specialization dynamics during spermatogenesis in the rat testis
|Li N,Mruk DD,Wong CK,Lee WM,Han D,Cheng CY||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (29:3788)||2015|
Axon behavior in the olfactory nerve reflects the involvement of catenin-cadherin mediated adhesion.
13-9700 was used in immunohistochemistry to examine the involvement of catenin-cadherin mediated adhesion in the axonal projection of olfactory sensory neuron (OSN) axons
|Akins MR,Greer CA||The Journal of comparative neurology (499:979)||2006|
|Human||Not Cited||α-Catenin contributes to the strength of E-cadherin-p120 interactions.||Troyanovsky RB,Klingelhöfer J,Troyanovsky SM||Molecular biology of the cell (22:4247)||2011|
|Human||Not Cited||Melanoma prognostic model using tissue microarrays and genetic algorithms.||Gould Rothberg BE,Berger AJ,Molinaro AM,Subtil A,Krauthammer MO,Camp RL,Bradley WR,Ariyan S,Kluger HM,Rimm DL||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (27:5772)||2009|
|Human||Not Cited||Protein expression profiling identifies subclasses of breast cancer and predicts prognosis.||Jacquemier J,Ginestier C,Rougemont J,Bardou VJ,Charafe-Jauffret E,Geneix J,Adélaïde J,Koki A,Houvenaeghel G,Hassoun J,Maraninchi D,Viens P,Birnbaum D,Bertucci F||Cancer research (65:767)||2005|
|Mouse||Not Cited||Progressive changes in adherens junction structure during intestinal adenoma formation in Apc mutant mice.||Carothers AM,Melstrom KA,Mueller JD,Weyant MJ,Bertagnolli MM||The Journal of biological chemistry (276:39094)||2001|
102 kDa cadherin-associated protein; 102kDa; alpha E catenin; alpha E-catenin; alpha(E)-catenin; alpha-catenin; alpha-E-catenin; alphaE-catenin; cadherin associated protein; cadherin-associated protein; CAP102; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin alpha 1; catenin alpha-1; renal carcinoma antigen NY-REN-13
2010010M04Rik; AA517462; AI988031; CAP102; Catna1; CTNNA1; MDPT2