Immunofluorescent analysis of alpha Catenin was performed using 90% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha Catenin (alpha-CAT-7A4) Mouse Monoclonal Antibody (13-9700) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cell junctional localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Chicken, Human, Mouse, Rat, Xenopus|
|Published species reactivity||Dog, Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide derived from the C-terminus of mouse alpha-catenin.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/mL|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 3 publications below|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|Immunofluorescence (IF)||See 2 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 2 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 4 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 2 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Western Blot (WB)||See 2 publications below|
Alpha catenin is a member of the b-catenin family of cytosolic proteins. Alpha catenin interacts with N-cadherin and b-catenin on the cytosolic side of adherins junctions to mediate the interaction with the actin cytoskeleton. Alpha catenin is a homodimer in solution, but dissociates to form a 1:1 heterodimer with b-catenin in order to interact with cadherins. Mice that have a-catenin ablated in their surface epithelium demonstrate reduced hair growth, multinucleated cells, extensive proliferation and a general cancerous phenotype.
IP-MS enrichment of CTNNA1 (LFQ intensity): CTNNA1 was enriched 118-fold from HEPG2 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and CTNNA1 antibody (Part No. 13-9700). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis.
13-9700 was used in immunohistochemistry to study the role of plastin 3 in ectoplasmic specialization dynamics during spermatogenesis in the rat testis
|Li N,Mruk DD,Wong CK,Lee WM,Han D,Cheng CY||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (29:3788)||2015|
Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*.
13-9700 was used in immunohistochemistry to report that Rai14 regulates actin filaments organization in Sertoli cells during the epithelial cycle
|Qian X,Mruk DD,Cheng CY||PloS one (8:null)||2013|
Axon behavior in the olfactory nerve reflects the involvement of catenin-cadherin mediated adhesion.
13-9700 was used in immunohistochemistry to examine the involvement of catenin-cadherin mediated adhesion in the axonal projection of olfactory sensory neuron (OSN) axons
|Akins MR,Greer CA||The Journal of comparative neurology (499:979)||2006|
Characterization of ¿-, ß- and p120-Catenin Expression in Feline Mammary Tissues and their Relation with E- and P-Cadherin.
13-9700 was used in immunohistochemistry (paraffin) to discuss catenin expression in feline mammary tissues.
|Figueira AC,Gomes C,Vilhena H,Miranda S,Carvalheira J,DE Matos AJ,Dias-Pereira P,Gärtner F||Anticancer research (35:3361)||2015|
Analysis of gene expression in human bullous keratopathy corneas containing limiting amounts of RNA.
13-9700 was used in immunohistochemistry (frozen) to validate the use of polymerase chain reaction-amplified full-length cDNA as a substitute for mRNA in nucleic acid array and gene expression analysis.
|Spirin KS,Ljubimov AV,Castellon R,Wiedoeft O,Marano M,Sheppard D,Kenney MC,Brown DJ||Investigative ophthalmology and visual science (40:3108)||1999|
Changing roles of cadherins and catenins during progression of squamous intraepithelial lesions in the uterine cervix.
13-9700 was used in western blot to elucidate the transformation of normal squamous epithelium to squamous intraepithelial lesions using the uterine cervix.
|de Boer CJ,van Dorst E,van Krieken H,Jansen-van Rhijn CM,Warnaar SO,Fleuren GJ,Litvinov SV||The American journal of pathology (155:505)||1999|
|Human||Not Cited||¿-Catenin contributes to the strength of E-cadherin-p120 interactions.||Troyanovsky RB,Klingelhöfer J,Troyanovsky SM||Molecular biology of the cell (22:4247)||2011|
||Distinct subcellular expression of endogenous polycystin-2 in the plasma membrane and Golgi apparatus of MDCK cells.||Scheffers MS,Le H,van der Bent P,Leonhard W,Prins F,Spruit L,Breuning MH,de Heer E,Peters DJ||Human molecular genetics (11:59)||2002|
|Human||Not Cited||Melanoma prognostic model using tissue microarrays and genetic algorithms.||Gould Rothberg BE,Berger AJ,Molinaro AM,Subtil A,Krauthammer MO,Camp RL,Bradley WR,Ariyan S,Kluger HM,Rimm DL||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (27:5772)||2009|
|Human||Not Cited||Protein expression profiling identifies subclasses of breast cancer and predicts prognosis.||Jacquemier J,Ginestier C,Rougemont J,Bardou VJ,Charafe-Jauffret E,Geneix J,Adélaïde J,Koki A,Houvenaeghel G,Hassoun J,Maraninchi D,Viens P,Birnbaum D,Bertucci F||Cancer research (65:767)||2005|
Cell discohesion and multifocality of carcinoma in situ of the bladder: new insight from the adhesion molecule profile (e-cadherin, Ep-CAM, and MUC1).
13-9700 was used in immunohistochemistry - paraffin section to determine the expression of several adhesion molecules and MUC1 in samples from patients with unifocal or multifocal bladder urothelial cell carcinoma in situ
|Patriarca C,Colombo P,Pio Taronna A,Wesseling J,Franchi G,Guddo F,Naspro R,Macchi RM,Giunta P,Di Pasquale M,Parente M,Arizzi C,Roncalli M,Campo B||International journal of surgical pathology (17:99)||2009|
Expression of the E-cadherin-catenins complex in sentinel node is related to tumor morphology but not to spread to nonsentinel nodes.
13-9700 was used in immunohistochemistry - paraffin section to quantify the expression of E-cadherin and a- and b-catenins that form the cadherin-catenin complex in tumoral vascular invasion in metastatic sentinel lymph node patients
|Canavese G,Bernardi A,Candelaresi G,Lovadina P,Amerio S,Rossetti V,Rabagliati C,Berardengo E||Pathology, research and practice (203:517)||2007|
Immunohistochemical patterns for alpha- and beta-catenin, E- and N-cadherin expression in ovarian epithelial tumors.
13-9700 was used in immunohistochemistry - paraffin section to measure the E- and N-cadherin and beta- and alpha-catenin expression in benign and malignant epithelial neoplasms of the ovary and correlate expression with tumor staging, histological grade,
|Marques FR,Fonsechi-Carvasan GA,De Angelo Andrade LA,Böttcher-Luiz F||Gynecologic oncology (94:16)||2004|
Abnormal expression of E-cadherin in early gastric carcinoma: its relationship with macroscopic growth patterns and catenin alpha and beta.
13-9700 was used in immunohistochemistry - paraffin section to elucidate the relation between the macroscopic growth patterns of gastric carcinoma and the abnormal expression of E-cadherin and alpha- and beta-catenins
|Song SY,Kim S,Kim DS,Son HJ,Rhee JC,Kim YI||Journal of clinical gastroenterology (38:252)||2004|
|Not Applicable||Not Cited||
Localization of claudin-5 and ZO-1 in rat spleen sinus endothelial cells.
13-9700 was used in immunohistochemistry - frozen section to study the spatial and functional relationships between tight and adherens junctions in the sinus endothelial cells of rat spleen
|Uehara K,Uehara A||Histochemistry and cell biology (129:95)||2008|
|Not Applicable||Not Cited||
Remodeling of myocyte gap junctions in arrhythmogenic right ventricular cardiomyopathy due to a deletion in plakoglobin (Naxos disease).
13-9700 was used in immunohistochemistry - frozen section to investigate the effect of the plakoglobin mutation in Naxos disease on connexin43
|Kaplan SR,Gard JJ,Protonotarios N,Tsatsopoulou A,Spiliopoulou C,Anastasakis A,Squarcioni CP,McKenna WJ,Thiene G,Basso C,Brousse N,Fontaine G,Saffitz JE||Heart rhythm (1:3)||2004|
|Not Applicable||Not Cited||
Modulating the strength of cadherin adhesion: evidence for a novel adhesion complex.
13-9700 was used in immunocytochemistry and western blot to identify and characterize a novel classical cadherin adhesion system
|Kim YJ,Sauer C,Testa K,Wahl JK,Svoboda RA,Johnson KR,Wheelock MJ,Knudsen KA||Journal of cell science (118:3883)||2005|
|Not Applicable||Not Cited||
Monocytes induce reversible focal changes in vascular endothelial cadherin complex during transendothelial migration under flow.
13-9700 was used in immunocytochemistry to propose that an endothelial-dependent step of transient and focal disruption of the vascular endothelial-cadherin complex occurs during leukocyte transmigration
|Allport JR,Muller WA,Luscinskas FW||The Journal of cell biology (148:203)||2000|
|Dog||Not Cited||Distinct subcellular expression of endogenous polycystin-2 in the plasma membrane and Golgi apparatus of MDCK cells.||Scheffers MS,Le H,van der Bent P,Leonhard W,Prins F,Spruit L,Breuning MH,de Heer E,Peters DJ||Human molecular genetics (11:59)||2002|
|Mouse||Not Cited||Progressive changes in adherens junction structure during intestinal adenoma formation in Apc mutant mice.||Carothers AM,Melstrom KA,Mueller JD,Weyant MJ,Bertagnolli MM||The Journal of biological chemistry (276:39094)||2001|