|Tested species reactivity||Bovine, Human, Mouse, Pig, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified recombinant rat a-internexin.|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:5000|
|Western Blot (WB)||1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The epitope recognized by the 2E3 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so this antibody is ideal for studies of formalin-fixed paraffin-embedded histological sections.
alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An autopsy case of neuronal intermediate filament inclusion disease with regard to immunophenotypic and topographical analysis of the neuronal inclusions.
MA3-16726 was used in immunohistochemistry - paraffin section to report an autopsy case of neuronal intermediate filament inclusion disease.
|Inoue K,Fujimura H,Ueda K,Matsumura T,Itoh K,Sakoda S||Neuropathology : official journal of the Japanese Society of Neuropathology (35:545)||2015|
Cytoplasmic non-epithelial mucin accumulation associated with CD44 in an astrocytic tumor with signet ring features.
MA3-16726 was used in immunohistochemistry to report on a case of an unusual astrocytic tumor rich in signet ring cells
|Okabe H,Nagata A,Katsura K,Ishida M,Osaka Y,Tenjin H||Brain tumor pathology (31:124)||2014|
Gliosarcoma with ependymal and PNET-like differentiation.
MA3-16726 was used in immunohistochemistry to characterize a rare case of gliosarcoma
|Shintaku M,Yoneda H,Hirato J,Nagaishi M,Okabe H||Clinical neuropathology (32:508)||2013|