|Tested species reactivity||Many|
|Published species reactivity||Pig, Zebrafish, Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified rabbit skeletal alpha-actinin.|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:25-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-22863 detects sarcomeric alpha Actinin in bovine, feline, chicken, fish, goat, hamster, lizard, mouse porcine, rabbit, rat, ovine, snake, and Xenopus laevis samples.
MA1-22863 has been successfully used in immunohistochemistry (frozen and paraffin) procedures.
The MA1-22863 immunogen is purified rabbit skeletal alpha-actinin.
This antibody is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish.
MA1-22863 was used in immunohistochemistry to characterize zebrafish with Titin truncations
|Zou J,Tran D,Baalbaki M,Tang LF,Poon A,Pelonero A,Titus EW,Yuan C,Shi C,Patchava S,Halper E,Garg J,Movsesyan I,Yin C,Wu R,Wilsbacher LD,Liu J,Hager RL,Coughlin SR,Jinek M,Pullinger CR,Kane JP,Hart DO,Kwok PY,Deo RC||eLife (4:null)||2015|
Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function.
MA1-22863 was used in immunocytochemistry to investigate the impact of Tmem65 on heart development
|Sharma P,Abbasi C,Lazic S,Teng AC,Wang D,Dubois N,Ignatchenko V,Wong V,Liu J,Araki T,Tiburcy M,Ackerley C,Zimmermann WH,Hamilton R,Sun Y,Liu PP,Keller G,Stagljar I,Scott IC,Kislinger T,Gramolini AO||Nature communications (6:null)||2015|
Dystrophin-deficient cardiomyocytes derived from human urine: new biologic reagents for drug discovery.
MA1-22863 was used in immunohistochemistry to study the generation of cardiomyocytes from human urine-derived stem cells obtained from a patient with Duchenne muscular dystrophy
|Guan X,Mack DL,Moreno CM,Strande JL,Mathieu J,Shi Y,Markert CD,Wang Z,Liu G,Lawlor MW,Moorefield EC,Jones TN,Fugate JA,Furth ME,Murry CE,Ruohola-Baker H,Zhang Y,Santana LF,Childers MK||Stem cell research (12:467)||2014|
Cyclin A2 induces cardiac regeneration after myocardial infarction through cytokinesis of adult cardiomyocytes.
MA1-22863 was used in immunocytochemistry and immunohistochemistry to study the role of adult cardiomyocyte cytokinesis in the mechanism by which adenoviral delivery of cyclin A2 to infarcted porcine heart promotes regeneration
|Shapiro SD,Ranjan AK,Kawase Y,Cheng RK,Kara RJ,Bhattacharya R,Guzman-Martinez G,Sanz J,Garcia MJ,Chaudhry HW||Science translational medicine (6:null)||2014|
Amniotic fluid stem cells morph into a cardiovascular lineage: analysis of a chemically induced cardiac and vascular commitment.
MA1-22863 was used in immunocytochemistry to study the ability of a mixture of hyaluronic, butyric and retinoic acids to promote the differentiation of amniotic stem cells towards a cardiovascular lineage
|Maioli M,Contini G,Santaniello S,Bandiera P,Pigliaru G,Sanna R,Rinaldi S,Delitala AP,Montella A,Bagella L,Ventura C||Drug design, development and therapy (7:1063)||2013|