Invitrogen

alpha Tubulin Polyclonal Antibody

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Antibody Testing Data (7)
Published Figures (2)

alpha Tubulin Antibody in Western Blot (WB)

FIGURE: 1 / 9

alpha Tubulin Antibody (PA5-16891) in WB

Western blot analysis was performed on whole cell extracts (30µg lysate) of SH-SY5Y (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), NIH/3T3 (Lane 4), MDA-MB-231 (Lane 5), L6 (Lane 6), RSC96 (Lane 7), tissue extracts of Mouse Lung (Lane 8) and Rat Brain (Lane 9). The blot was probed with Anti- alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-16891, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). A 52 kDa band cor... View More

Product Image Gallery

alpha Tubulin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Tubulin alpha was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Tubulin alpha Rabbit Polyclonal Antibody (Product # PA5-16891) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

alpha Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Tubulin alpha showing staining in the cytoplasm of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Tubulin alpha Rabbit Polyclonal Antibody (Product # PA5-16891) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Product Details

PA5-16891

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

2 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide derived from aa 426-450 of human tubulin alpha

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10984790

Product Specific Information

PA5-16891 targets Tubulin alpha in immunohistochemistry (paraffin) and Western blot applications and shows reactivity with Bovine, Chicken, amphibian, Gerbil, Guinea Pig, Human, mouse, Porcine, and Rat samples.

The PA5-16891 immunogen is a synthetic peptide derived from aa 426-450 of human tubulin alpha.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Molecular Features of Polycystic Ovary Syndrome Revealed by Transcriptome Analysis of Oocytes and Cumulus Cells.

Frontiers in cell and developmental biology

Li J,Chen H,Gou M,Tian C,Wang H,Song X,Keefe DL,Bai X,Liu L

Published figure using alpha Tubulin polyclonal antibody (Product # PA5-16891) in Immunocytochemistry

Sat Sep 25 00:00:00 EDT 2021

Adipose gene expression profiles reveal insights into the adaptation of northern Eurasian semi-domestic reindeer (Rangifer tarandus).

Communications biology

Weldenegodguad M,Pokharel K,Niiranen L,Soppela P,Ammosov I,Honkatukia M,Lindeberg H,Peippo J,Reilas T,Mazzullo N,Mäkelä KA,Nyman T,Tervahauta A,Herzig KH,Stammler F,Kantanen J

Published figure using alpha Tubulin polyclonal antibody (Product # PA5-16891) in Western Blot

Thu Oct 07 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: alpha tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 6; Alpha-tubulin ubiquitous; class II beta-tubulin isotype; class IIb beta-tubulin; Detyrosinated tubulin alpha-1A chain; Detyrosinated tubulin alpha-1B chain; Detyrosinated tubulin alpha-1C chain; Detyrosinated tubulin alpha-3C chain; Detyrosinated tubulin alpha-3E chain; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2B class IIb; tubulin, beta polypeptide paralog

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Gene Aliases: ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; H2-ALPHA; K-ALPHA-1; LIS3; PMGYSA; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; TUBA4A; TUBA6; TUBAL3; TUBB2B

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UniProt ID: (Human) Q71U36, (Human) P68363, (Human) Q9BQE3, (Human) P0DPH7, (Human) Q6PEY2, (Human) P68366, (Human) A6NHL2, (Human) Q9BVA1

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Entrez Gene ID: (Human) 7846, (Human) 10376, (Human) 84790, (Human) 7278, (Human) 112714, (Human) 7277, (Human) 79861, (Human) 347733

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Molecular Function: tubulin

Function(s)

G2/M transition of mitotic cell cycle cytoskeleton organization microtubule-based process cytoskeleton-dependent intracellular transport cell division microtubule cytoskeleton organization cellular response to interleukin-4 biological_process platelet degranulation neuron migration

Process(es)

GTPase activity structural molecule activity structural constituent of cytoskeleton protein binding GTP binding protein domain specific binding double-stranded RNA binding ubiquitin protein ligase binding molecular_function enzyme binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Tubulin Polyclonal Antibody

alpha Tubulin Antibody (PA5-16891) in WB

Product Details

PA5-16891

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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