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alpha Tubulin Polyclonal Antibody

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Catalog # PA5-16891

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alpha Tubulin Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of Tubulin alpha was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Tubulin alpha Rabbit Polyclonal Antibody (Product # PA5-16891) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

alpha Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Tubulin alpha showing staining in the cytoplasm of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Tubulin alpha Rabbit Polyclonal Antibody (Product # PA5-16891) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

alpha Tubulin Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human lung stained with Tubulin alpha antibody using peroxidase-conjugate and AEC. Note cytoplasmic staining of ciliated epithelium of bronchioles.

alpha Tubulin Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30µg lysate) of SH-SY5Y (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), NIH/3T3 (Lane 4), MDA-MB-231 (Lane 5), L6 (Lane 6), RSC96 (Lane 7), tissue extracts of Mouse Lung (Lane 8) and Rat Brain (Lane 9). The blot was probed with Anti- alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-16891, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). A 52 kDa band corresponding to alpha Tubulin was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

TESTED APPLICATIONS	DILUTION
Immunocytochemistry (ICC)	2 µg/ml
Immunofluorescence (IF)	2 µg/ml
Immunohistochemistry (Paraffin) (IHC (P))	1:100
Western Blot (WB)	1:250

Species reactivity	Amphibian, Bovine, Dog, Chicken, Gerbil, Guinea pig, Human, Mouse, Non-human primate, Pig, Rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide derived from aa 426-450 of human tubulin alpha
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/ml
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.6, with 0.2% BSA
Contains	15mM sodium azide
Storage conditions	4° C
RRID	AB_10984790

Product Specific Information

PA5-16891 targets Tubulin alpha in immunohistochemistry (paraffin) and Western blot applications and shows reactivity with Bovine, Chicken, amphibian, Gerbil, Guinea Pig, Human, mouse, Porcine, and Rat samples.

The PA5-16891 immunogen is a synthetic peptide derived from aa 426-450 of human tubulin alpha.

Background/Target Information

Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kDa subunits: alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha; alpha 3; Alpha-tubulin 1; Alpha-tubulin 3; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-6; alpha-tubulin ubiquitous; brain-specific; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; tubulin; tubulin alpha 6; Tubulin alpha-1 chain; Tubulin alpha-1A chain; tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-3 chain; Tubulin alpha-6 chain; tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1c; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific

Gene Aliases: B-ALPHA-1; bcm948; BOS_5053; BOS_5055; LIS3; M[a]6; Tuba-1; TUBA1; TUBA1A; TUBA1C; TUBA3; TUBA4A; TUBA6

UniProt ID: (Human) Q71U36, (Bovine) Q3ZCJ7, (Human) Q9BQE3, (Mouse) P68369, (Rat) P68370, (Mouse) P68373

Entrez Gene ID: (Bovine) 404053, (Human) 7846, (Bovine) 504244, (Human) 84790, (Chicken) 429035, (Mouse) 22142, (Rat) 64158, (Rat) 300218, (Mouse) 22146

Molecular Function: cytoskeletal protein microtubule family cytoskeletal protein tubulin

Function(s)

microtubule cytoskeleton organization protein polymerization cellular response to interleukin-4 G2/M transition of mitotic cell cycle cytoskeleton organization microtubule-based process cytoskeleton-dependent intracellular transport cell division metabolic process biological_process

Process(es)

GTPase activity
structural constituent of cytoskeleton
GTP binding
structural molecule activity
protein binding
protein domain specific binding
nucleotide binding
protein heterodimerization activity
molecular_function

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alpha Tubulin Polyclonal Antibody

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alpha Tubulin Polyclonal Antibody

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Product Details

Product Specific Information

Background/Target Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

We’re here to help

Brands

Shopping Tool

eSolutions

Support

Thermo Fisher Scientific

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