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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • alpha Tubulin Antibodies

          Invitrogen

          alpha Tubulin Polyclonal Antibody

          2 Published Figures
          2 References
          View all (75) alpha Tubulin antibodies
          Datasheet
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          Tech Support

          Cite alpha Tubulin Polyclonal Antibody

          Datasheet
          Protocols
          Questions & Answers
          Tech Support
          • Antibody Testing Data (7)
          • Published Figures (2)
          alpha Tubulin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          alpha Tubulin Antibody (PA5-16891) in WB

          Western blot analysis was performed on whole cell extracts (30µg lysate) of SH-SY5Y (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), NIH/3T3 (Lane 4), MDA-MB-231 (Lane 5), L6 (Lane 6), RSC96 (Lane 7), tissue extracts of Mouse Lung (Lane 8) and Rat Brain (Lane 9). The blot was probed with Anti- alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-16891, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). A 52 kDa band cor... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Product Details

          PA5-16891

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:250
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100
          -

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Not Applicable

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic peptide derived from aa 426-450 of human tubulin alpha
          View immunogen

          Conjugate

          Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.6, with 1% BSA

          Contains

          0.1% sodium azide

          Storage conditions

          -20° C, Avoid Freeze/Thaw Cycles

          RRID

          AB_10984790

          Product Specific Information

          PA5-16891 targets Tubulin alpha in immunohistochemistry (paraffin) and Western blot applications and shows reactivity with Bovine, Chicken, amphibian, Gerbil, Guinea Pig, Human, mouse, Porcine, and Rat samples.

          The PA5-16891 immunogen is a synthetic peptide derived from aa 426-450 of human tubulin alpha.

          Target Information

          Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: alpha tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 6; Alpha-tubulin ubiquitous; class II beta-tubulin isotype; class IIb beta-tubulin; Detyrosinated tubulin alpha-1A chain; Detyrosinated tubulin alpha-1B chain; Detyrosinated tubulin alpha-1C chain; Detyrosinated tubulin alpha-3C chain; Detyrosinated tubulin alpha-3E chain; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2B class IIb; tubulin, beta polypeptide paralog

          View more View less

          Gene Aliases: ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; H2-ALPHA; K-ALPHA-1; LIS3; PMGYSA; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; TUBA4A; TUBA6; TUBAL3; TUBB2B

          View more View less

          UniProt ID: (Human) Q71U36, (Human) P68363, (Human) Q9BQE3, (Human) P0DPH7, (Human) Q6PEY2, (Human) P68366, (Human) A6NHL2, (Human) Q9BVA1

          View more View less

          Entrez Gene ID: (Human) 7846, (Human) 10376, (Human) 84790, (Human) 7278, (Human) 112714, (Human) 7277, (Human) 79861, (Human) 347733

          View more View less

          Molecular Function: tubulin

          Function(s)
          G2/M transition of mitotic cell cycle cytoskeleton organization microtubule-based process cytoskeleton-dependent intracellular transport cell division microtubule cytoskeleton organization cellular response to interleukin-4 biological_process platelet degranulation neuron migration
          Process(es)
          GTPase activity structural molecule activity structural constituent of cytoskeleton protein binding GTP binding protein domain specific binding double-stranded RNA binding ubiquitin protein ligase binding molecular_function enzyme binding
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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