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Immunofluorescent analysis of Phalloidin (dark red) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 680 Phalloidin (Product # 21839) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
|Tested species reactivity||Many|
|Published species reactivity||Bovine, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1:100 - 1:500|
|Immunofluorescence (IF)||1:100 - 1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
HPB-ALL human peripheral blood leukemia cell line is the suggested positive control (incubate for 90 mins).
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
Tubulin is a heterodimer consisting of alpha and beta-tubulin subunits. It is nearly ubiquitous among eukaryotic cells. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules. This intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Sera from early-onset, severely preeclamptic women directly modulate HLA-E expression in the EA.hy296 endothelial cell line.
MA1-19162 was used in western blot to study the modulation of endothelial cell line expression of HLA-E by sera obtained from women with severe early onset preeclampsia
|Bueno-Sánchez JC,Peña-Alzate S,Peña RB,Agudelo-Jaramillo B,Cadavid-Jaramillo AP,Chaouat G,Maldonado-Estrada JG||Journal of reproductive immunology (104-105:68)||2014|
Proteinase-activated receptor 2 expression in the intestinal tract of the horse.
MA1-19162 was used in western blot to study the equine intestinal expression of PAR2
|Zannoni A,Bombardi C,Dondi F,Morini M,Forni M,Chiocchetti R,Spadari A,Romagnoli N||Research in veterinary science (96:464)||2014|
Methoxychlor and triclosan stimulates ovarian cancer growth by regulating cell cycle- and apoptosis-related genes via an estrogen receptor-dependent pathway.
MA1-19162 was used in western blot to study the role of ER-dependent modulation of cell cycle and apoptosis genes in the mechanism by which methoxychlor and triclosan promote the growth of ovarian cancer
|Kim JY,Yi BR,Go RE,Hwang KA,Nam KH,Choi KC||Environmental toxicology and pharmacology (37:1264)||2014|
Epidermal Snail expression drives skin cancer initiation and progression through enhanced cytoprotection, epidermal stem/progenitor cell expansion and enhanced metastatic potential.
MA1-19162 was used in immunohistochemistry to investigate the role of Snail during progression of epithelial cancer
|De Craene B,Denecker G,Vermassen P,Taminau J,Mauch C,Derore A,Jonkers J,Fuchs E,Berx G||Cell death and differentiation (21:310)||2014|
alpha-tubulin 1; alpha-tubulin 4; alpha-tubulin isotype M-alpha-4; FLJ30169; H2 ALPHA; Testis specific alpha tubulin; TUBA1; Tubulin alpha; tubulin alpha-1 chain; tubulin alpha-4 chain; tubulin alpha-4A chain; tubulin H2-alpha; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 4; tubulin, alpha 4a
ALS22; BOS_2117; H2-ALPHA; M[a]4; TUBA1; Tuba4; TUBA4A