|Immunocytochemistry (ICC)||1:100 - 1:500|
|Immunofluorescence (IF)||1:100 - 1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1-2 µg/ml|
|Western Blot (WB)||See 13 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 9 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Many|
|Published species reactivity||Rat , Non-human primate , Bovine , Human , Mouse , Xenopus|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
HPB-ALL human peripheral blood leukemia cell line is the suggested positive control (incubate for 90 mins).
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
Tubulin is a heterodimer consisting of alpha and beta-tubulin subunits. It is nearly ubiquitous among eukaryotic cells. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules. This intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alpha-tubulin 1; Alpha-tubulin 4; Alpha-tubulin isotype M-alpha-4; FLJ30169; H2 ALPHA; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA1; Tubulin alpha; Tubulin alpha-1 chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin H2-alpha; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 4; tubulin, alpha 4a
Gene Aliases: ALS22; BOS_2117; H2-ALPHA; M[a]4; TUBA1; Tuba4; TUBA4A
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