Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Western blot analysis of adiponectin was performed by loading 8.4ug of rabbit subcutaneous white adipose tissue (scWAT), gonadal white adipose tissue (gWAT), perirenal white adipose tissue (pWAT), tibia marrow adipose tissue (tibMAT), tibia red marrow (tibRM), and 3T3-L1 positive control adipocyte lysates per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with an adiponectin monoclonal antibody (Product # MA1-054) at a dilution of 1:1000 in 2.5% milk in TBST for 16 h at 4°C, washed in TBST, and probed with an HRP-conjugated mouse IgG secondary antibody at a dilution of 1:5000 for 1 hour at room temperature. As a loading control, lysates were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-80017) at a dilution of 1:2000, followed by an HRP-conjugated rat IgG secondary antibody at a dilution of 1:5000 for 1 hour at room temperature. Detection was performed using a chemiluminescent substrate. Data courtesy of the Innovators Program.
|Tested species reactivity||Bovine, Dog, Fruit fly, Human, Mouse, Primate, Plant, Pig, Rabbit, Rat, Clawed frog, Yeast|
|Published species reactivity||Dog, Rat|
|Host / Isotype||Rat / IgG2a|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1/100 - 1/1000|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Radioimmune Assays (RIA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This clone recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this antibody appears to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).
MA1-80017 is predicted to react with amphibia, birds, and echinoderm based on sequence homology. Does not react with nephrotoma suturalis.
This antibody is suitable for use as a loading control.The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin.
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation.
MA1-80017 was used in immunocytochemistry to study the role of MAL, a tetraspanning protein, in primary cilium formation
|Reales E,Bernabé-Rubio M,Casares-Arias J,Rentero C,Fernández-Barrera J,Rangel L,Correas I,Enrich C,Andrés G,Alonso MA||Journal of cell science (128:2261)||2015|
Wnt6, Wnt10a and Wnt10b inhibit adipogenesis and stimulate osteoblastogenesis through a β-catenin-dependent mechanism.
MA1-80017 was used in western blot to study the mechanism for the effects of Wnt6, Wnt10a and Wnt10b on adipogenesis and osteoblastogenesis
|Cawthorn WP,Bree AJ,Yao Y,Du B,Hemati N,Martinez-Santibañez G,MacDougald OA||Bone (50:477)||2012|
alpha-tubulin 1; alpha-tubulin isotype M-alpha-1; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; Tuba1; tubulin alpha-1 chain; tubulin alpha-1A chain; tubulin alpha-3 chain; tubulin B-alpha-1; tubulin, alpha 1; tubulin, alpha 1a; tubulin, alpha, brain-specific
B-ALPHA-1; LIS3; Tuba-1; Tuba1; TUBA1A; TUBA3