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Immunofluorescence analysis of Alpha-Tubulin was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Alpha-Tubulin Mouse monoclonal Antibody (A11126) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Bovine, Human, Mouse|
|Published species reactivity||Rat, Primate, Bovine, Hamster, Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||1:1000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Storage and reconstitution: upon receipt, store desiccated at -20°C. To prepare a stock solution, reconstitute in PBS containing 1% BSA and 2mM sodium azide. Reconstituted solutions should remain stable for 6 months when stored at 4°C. For longer storage, aliquot and store at -20°C.
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mcl-1 is a key regulator of the ovarian reserve.
A11126 was used in immunocytochemistry to investigate the role of MCL-1 in oocyte survival
|Omari S,Waters M,Naranian T,Kim K,Perumalsamy AL,Chi M,Greenblatt E,Moley KH,Opferman JT,Jurisicova A||Cell death & disease (6:null)||2015|
Mitochondrial dysfunction in oocytes of obese mothers: transmission to offspring and reversal by pharmacological endoplasmic reticulum stress inhibitors.
A11126 was used in immunocytochemistry to show that obesity before conception imparts a reversible legacy of mitochondrial loss in offspring that is caused by ER stress
|Wu LL,Russell DL,Wong SL,Chen M,Tsai TS,St John JC,Norman RJ,Febbraio MA,Carroll J,Robker RL||Development (Cambridge, England) (142:681)||2015|
Reprogramming cellular phenotype by soft collagen gels.
A11126 was used in immunocytochemistry to study the effect of the mechanical stiffness of the substrate on cell phenotypes
|Ali MY,Chuang CY,Saif MT||Soft matter (10:8829)||2014|
Adenomatous polyposis coli regulates oligodendroglial development.
A11126 was used in immunocytochemistry to study the mechanisms by which the the gut tumor suppressor APC regulates the development of oligodendroglia
|Lang J,Maeda Y,Bannerman P,Xu J,Horiuchi M,Pleasure D,Guo F||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:3113)||2013|
Estrous cycle-dependent changes of Fas expression in the bovine corpus luteum: influence of keratin 8/18 intermediate filaments and cytokines.
A11126 was used in immunocytochemistry to investigate the effect of keratin 8/18 intermediate filaments and cytokines on Fas expression in the bovine corpus luteum
|Duncan A,Forcina J,Birt A,Townson D||Reproductive biology and endocrinology : RB&E (10:null)||2013|
Timing of anaphase-promoting complex activation in mouse oocytes is predicted by microtubule-kinetochore attachment but not by bivalent alignment or tension.
A11126 was used in immunocytochemistry to study the role of stable kinetochore-microtubule attachments on aneuploidy.
|Lane SI,Yun Y,Jones KT||Development (Cambridge, England) (139:1947)||2012|
Centrosomal dysregulation in human metastatic melanoma cell lines.
A11126 was used in immunocytochemistry to determine the centrosomal status, ploidy, and gene status of 5 human metastatic melanoma cell lines.
|Charters GA,Stones CJ,Shelling AN,Baguley BC,Finlay GJ||Cancer genetics (204:477)||2011|
Essential role of protein phosphatase 2A in metaphase II arrest and activation of mouse eggs shown by okadaic acid, dominant negative protein phosphatase 2A, and FTY720.
A11126 was used in immunocytochemistry to report that PP2A is needed for both metaphase arrest and successful exit from meiosis.
|Chang HY,Jennings PC,Stewart J,Verrills NM,Jones KT||The Journal of biological chemistry (286:14705)||2011|
Corticotropin releasing factor-induced CREB activation in striatal neurons occurs via a novel Gβγ signaling pathway.
A11126 was used in immunocytochemistry to elucidate the signaling pathway by which corticotropin-releasing factor activates CREB in striatal neurons.
|Stern CM,Luoma JI,Meitzen J,Mermelstein PG||PloS one (6:null)||2011|
Characterization of the mitofusin 2 R94W mutation in a knock-in mouse model.
A11126 was used in western blot to describe a mouse model that has many features of Charcot-Marie-Tooth disease
|Strickland AV,Rebelo AP,Zhang F,Price J,Bolon B,Silva JP,Wen R,Züchner S||Journal of the peripheral nervous system : JPNS (19:152)||2014|
Epithelial and stromal cells of bovine endometrium have roles in innate immunity and initiate inflammatory responses to bacterial lipopeptides in vitro via Toll-like receptors TLR2, TLR1, and TLR6.
A11126 was used in western blot to study the role of TLR-, -2 and -6 on bovine endometrial stromal and epithelial cells in the immune and inflammatory responses to bacterial lipopeptides.
|Turner ML,Cronin JG,Healey GD,Sheldon IM||Endocrinology (155:1453)||2014|
Atypical protein kinase Cι is required for Wnt3a-dependent neurite outgrowth and binds to phosphorylated dishevelled 2.
A11126 was used in western blot to study Wnt3a-dependent neurite outgrowth.
|Greer YE,Fields AP,Brown AM,Rubin JS||The Journal of biological chemistry (288:9438)||2013|
Stat1 activation attenuates IL-6 induced Stat3 activity but does not alter apoptosis sensitivity in multiple myeloma.
A11126 was used in western blot to elucidate the role of Stat in multiple myeloma.
|Dimberg LY,Dimberg A,Ivarsson K,Fryknäs M,Rickardson L,Tobin G,Ekman S,Larsson R,Gullberg U,Nilsson K,Öberg F,Wiklund HJ||BMC cancer (12:null)||2012|
The mechanical behavior of mutant K14-R125P keratin bundles and networks in NEB-1 keratinocytes.
A11126 was used in western blot to test if mutant K4-R25P filaments in human keratinocytes are mechanically defective in response to deformations.
|Beriault DR,Haddad O,McCuaig JV,Robinson ZJ,Russell D,Lane EB,Fudge DS||PloS one (7:null)||2012|
Characterization of the role of COP9 signalosome in regulating cullin E3 ubiquitin ligase activity.
A11126 was used in western blot to elucidate the mechanism through which COP9 signalosome promotes cullin RING ligase activity in vivo.
|Choo YY,Boh BK,Lou JJ,Eng J,Leck YC,Anders B,Smith PG,Hagen T||Molecular biology of the cell (22:4706)||2011|
An overlapping reading frame in the PRNP gene encodes a novel polypeptide distinct from the prion protein.
A11126 was used in western blot to study the expression and function of overlapping open reading frames in the prion protein gene PRNP.
|Vanderperre B,Staskevicius AB,Tremblay G,McCoy M,O'Neill MA,Cashman NR,Roucou X||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (25:2373)||2011|
Neddylation-induced conformational control regulates cullin RING ligase activity in vivo.
A11126 was used in western blot to study the effect of neddylation on Cul2 and Cul3 structure.
|Boh BK,Smith PG,Hagen T||Journal of molecular biology (409:136)||2011|
Regulation of cullin RING E3 ubiquitin ligases by CAND1 in vivo.
A11126 was used in western blot to suggest that CAND1 does not sequester cullins in vivo to prevent substrate receptor autoubiquitination.
|Chua YS,Boh BK,Ponyeam W,Hagen T||PloS one (6:null)||2011|
Pharmacologic suppression of JAK1/2 by JAK1/2 inhibitor AZD1480 potently inhibits IL-6-induced experimental prostate cancer metastases formation.
A11126 was used in immunohistochemistry to evaluate the effect of JAK1/2 inhibitor AZD1480 on IL-6-induced experimental prostate cancer metastases formation
|Gu L,Talati P,Vogiatzi P,Romero-Weaver AL,Abdulghani J,Liao Z,Leiby B,Hoang DT,Mirtti T,Alanen K,Zinda M,Huszar D,Nevalainen MT||Molecular cancer therapeutics (13:1246)||2014|
A unique ball-shaped Golgi apparatus in the rat pituitary gonadotrope: its functional implications in relation to the arrangement of the microtubule network.
A11126 was used in immunohistochemistry to determine the morphological characteristics of the Golgi apparatus in non-polarized endocrine cells.
|Watanabe T,Sakai Y,Koga D,Bochimoto H,Hira Y,Hosaka M,Ushiki T||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (60:588)||2012|
Effects of dual targeting of tumor cells and stroma in human glioblastoma xenografts with a tyrosine kinase inhibitor against c-MET and VEGFR2.
A11126 was used in western blot to study the therapeutic efficacy of a tyrosine kinase inhibitor active against both c-MET and VEGFR2 in a xenoraft model of human glioblastoma.
|Navis AC,Bourgonje A,Wesseling P,Wright A,Hendriks W,Verrijp K,van der Laak JA,Heerschap A,Leenders WP||PloS one (8:null)||2013|
A spatio-temporal analysis of matrix protein and nucleocapsid trafficking during vesicular stomatitis virus uncoating.
A11126 was used in immunocytochemistry and western blot to investigate the fate of incoming matrix protein from VSV during virus uncoating
|Mire CE,White JM,Whitt MA||PLoS pathogens (6:null)||2010|
Temperature-dependent arrest of neutrophil apoptosis. Failure of Bax insertion into mitochondria at 15 degrees C prevents the release of cytochrome c.
A11126 was used in western blot to study neutrophil apoptosis.
|Pryde JG,Walker A,Rossi AG,Hannah S,Haslett C||The Journal of biological chemistry (275:33574)||2000|
alpha 1; alpha-tubulin 1; alpha-tubulin 4; alpha-tubulin isotype M-alpha-4; H2-ALPHA; TUBA1; tubulin; tubulin alpha-1 chain; tubulin alpha-4 chain; tubulin alpha-4A chain; tubulin H2-alpha; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 4; tubulin, alpha 4a
ALS22; BOS_2117; H2-ALPHA; M[a]4; TUBA1; Tuba4; TUBA4A