The anti-bovine alpha-tubulin antibody can be used with secondary detection techniques to visualize microtubules in fixed cells and in fixed or frozen tissue sections of various species. In addition, this antibody can be used to detect tubulin by ELISA and immunoblot, for the screening of expression libraries and, since it recognizes amino acids 65-97 of the N-terminal structural domain, as a probe for the N-terminal domain of alpha-tubulin.
We have found that this highly specific antibody can be used at much lower concentrations than anti-tubulin antibodies available from other suppliers. For immunocytochemistry applications, we recommend using an initial concentration of 1 µg/mL or less.
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
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Protein Aliases: alpha tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; Detyrosinated tubulin alpha-1A chain; Detyrosinated tubulin alpha-1B chain; Detyrosinated tubulin alpha-1C chain; Detyrosinated tubulin alpha-3 chain; Detyrosinated tubulin alpha-3C chain; Detyrosinated tubulin alpha-3D chain; Detyrosinated tubulin alpha-3E chain; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; tubulin alpha 6; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3D chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3d; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous
Gene Aliases: ALS22; B-ALPHA-1; bA408E5.3; bcm948; BOS_16828; BOS_16831; BOS_2117; BOS_5053; BOS_5055; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3D; TUBA3E; Tuba4; TUBA4A; TUBA6
UniProt ID: (Human) Q71U36, (Bovine) P81947, (Human) P68363, (Human) Q9BQE3, (Bovine) Q3ZCJ7, (Human) P0DPH7, (Human) Q6PEY2, (Bovine) Q32KN8, (Human) P68366, (Bovine) P81948, (Mouse) P68369, (Mouse) P05213, (Mouse) P68373, (Mouse) P68368
Entrez Gene ID: (Bovine) 404053, (Human) 7846, (Bovine) 539882, (Human) 10376, (Human) 84790, (Bovine) 504244, (Human) 7278, (Human) 112714, (Bovine) 534900, (Human) 7277, (Bovine) 777775, (Mouse) 22142, (Mouse) 22143, (Mouse) 22146, (Mouse) 22145