|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Immunomicroscopy (IM)||Assay Dependent|
|Immunoprecipitation (IP)||2 ug/mg|
|Western Blot (WB)||1 µg/ml|
|Western Blot (WB)||See 76 publications below|
|Immunocytochemistry (ICC)||See 8 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Tested Species reactivity||Amphibian, Bovine, Dog, Chicken, Gerbil, Guinea pig, Human, Mouse, Non-human primate, Pig, Rat|
|Published species reactivity||Rat , Fruit fly , Non-human primate , Sheep , Hamster , Human , Mouse , Xenopus|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Native chick brain microtubules|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
62204 detects alpha tubulin in human, mouse, rat, bovine, porcine, chicken, amphibian, gerbil and guinea pig samples.
62204 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, electron microscopy, immunocytochemistry and immunofluorescent applications.
The 62204 immunogen is native chick brain microtubules. This antibody recognizes an epitope within amino acids 426-450 of alpha tubulin.
Tubulin is a heterodimer consisting of alpha and beta-tubulin subunits. It is nearly ubiquitous among eukaryotic cells. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules. This intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha 1; Alpha-tubulin 1; Alpha-tubulin 4; Alpha-tubulin isotype M-alpha-4; H2-ALPHA; Testis-specific alpha-tubulin; TUBA1; tubulin; Tubulin alpha-1 chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin H2-alpha; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 4; tubulin, alpha 4a
Gene Aliases: ALS22; BOS_2117; H2-ALPHA; M[a]4; TUBA1; Tuba4; TUBA4A
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