OriGene

alpha-actinin (Actinin alpha 1) Monoclonal Antibody (OTI7A4), TrueMAB™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

View all (18) alpha Actinin 1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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alpha-actinin (Actinin alpha 1) Antibody in Immunocytochemistry (ICC/IF) — Anti-ACTN1 mouse monoclonal antibody (TA500072) Immunofluorescent staining of Hela cells transiently transfected by pCMV6-ENTRY ACTN1(RC204924).

alpha-actinin (Actinin alpha 1) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human bladder tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500072)

alpha-actinin (Actinin alpha 1) Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACTN1 (RC204924, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ACTN1. Positive lysates LY400447 (100 µg) and LC400447 (20 µg) can be purchased separately from OriGene.

alpha-actinin (Actinin alpha 1) Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in ACTN1 knockout (KO) HeLa cell line using Anti-ACTN1 monoclonal antibody (Product # CF500072). {KO}

Product Details

CF500072

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI7A4
Immunogen	Human recombinant protein fragment corresponding to amino acids 650-893 of human alpha-actinin produced in E.coli. View immunogen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Alpha Actinin 1 belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: actinin 1 smooth muscle; actinin, alpha 1; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; F-actin cross-linking protein; FLJ40884; FLJ54432; Non-muscle alpha-actinin-1

Gene Aliases: ACTN1; BDPLT15

UniProt ID: (Human) P12814

Entrez Gene ID: (Human) 87

Function(s)

platelet degranulation actin filament organization platelet activation platelet formation platelet morphogenesis regulation of apoptotic process focal adhesion assembly actin filament bundle assembly negative regulation of cellular component movement actin filament network formation actin crosslink formation platelet aggregation regulation of nucleic acid-templated transcription

Process(es)

double-stranded RNA binding integrin binding calcium ion binding protein binding vinculin binding ligand-dependent nuclear receptor transcription coactivator activity protein homodimerization activity ion channel binding actin filament binding

alpha-actinin (Actinin alpha 1) Monoclonal Antibody (OTI7A4), TrueMAB™

Product Details

CF500072

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

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