Immunofluorescent analysis of alpha sarcomeric Actin showing staining in the cytoskeleton of HeLa cells. HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min and stained using an Alpha sarcomeric Actin polyclonal antibody (Product # PA5-21396) diluted at 1:200. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment contain a sequence corresponding to a region within amino acids 99 and 354 (P68032) of alpha Actin (cardiac muscle)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-21396 targets alpha sarcomeric Actin in IHC (P), IF, and WB applications and shows reactivity with Human, Rat, and mouse samples.
The PA5-21396 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 99 and 354 (P68032) of alpha Actin (cardiac muscle).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ascribing novel functions to the sarcomeric protein, myosin binding protein H (MyBPH) in cardiac sarcomere contraction.
PA5-21396 was used in immunocytochemistry, immunoprecipitation, and western blot to investigate the role of myosin binding protein H in hypertrophic cardiomyopathy
|Mouton J,Loos B,Moolman-Smook JC,Kinnear CJ||Experimental cell research (331:338)||2015|