Immunofluorescent staining of HeLa cells using PA5-19465, anti-Alpha smooth muscle Actin antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 20 minutes and exposed to the primary antibody at a concentration of 10 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
|Tested species reactivity||Horse, Human, Mouse, Rat, Zebrafish|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from within residues 1 - 100 of Human alpha smooth muscle Actin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||1:200-1:1000|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with rabbit, chicken and cow based on sequence homology.
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity.
PA5-19465 was used in immunocytochemistry to examine how the elasticity of the matrix affects the pluripotency of hPSCs
|Higuchi A,Kao SH,Ling QD,Chen YM,Li HF,Alarfaj AA,Munusamy MA,Murugan K,Chang SC,Lee HC,Hsu ST,Kumar SS,Umezawa A||Scientific reports (5:null)||2015|
Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway.
PA5-19465 was used in western blot to investigate the effect of FGF2 in mesenchymal stem cells
|Cai TY,Zhu W,Chen XS,Zhou SY,Jia LS,Sun YQ||Molecular medicine reports (8:1323)||2013|
Spirulina improves non-alcoholic steatohepatitis, visceral fat macrophage aggregation, and serum leptin in a mouse model of metabolic syndrome.
PA5-19465 was used in immunohistochemistry to study the beneficial effects of spirulina on macrophages and metabolic parameters in a murine model of metabolic syndrome
|Fujimoto M,Tsuneyama K,Fujimoto T,Selmi C,Gershwin ME,Shimada Y||Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver (44:767)||2012|