|Tested species reactivity||Bovine, Human, Rat|
|Published species reactivity||Bovine|
|Host / Isotype||Mouse / IgG1|
|Immunogen||AP2 adaptor from bovine brain.|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA1-25065 detects beta 1 + 2 Adaptin from human, rat, and bovine samples.
MA1-25065 has been successfully used in immunofluorescence and Western blot procedures. This antibody stains clathrin-coated membranes at the plasma membrane, and in the Golgi region of methanol-fixed MDBK cells. By Western bloy on bovine and rat brain preparations, this antibody detects a major band of AP-1 at ~115 kDa and a minor component at ~110 kDa, and bands at ~104 and ~112 kDa representing AP2. The antibody reacts with polypeptides at ~100 kDa in bovine liver, human heart fibroblasts and MDBK cells, but not with any components in the 110-115 kDa range from these sources, or from rat PC12 cells, neuroblastoma or astrocytes.
The MA1-25065 immunogen is AP2 adaptor from bovine brain.
Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
100-kD proteins of Golgi- and trans-Golgi network-associated coated vesicles have related but distinct membrane binding properties.
MA1-25065 was used in immunocytochemistry to study coat protein membrane binding properties in the Golgi region
|Wong DH,Brodsky FM||The Journal of cell biology (117:1171)||1992|