Immunofluorescent analysis of beta-1 Tubulin in HeLa cells, fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Polyethylene glycol tert-octylphenyl ether, using a beta-1 Tubulin monoclonal antibody (Product # MA1-25056) at a dilution of 1:5000 followed by detection using a Goat anti-Mouse IgG, Atto-488 conjugate. Nuclear were stained using DAPI (blue).
|Tested species reactivity||Bovine, Dog, Hamster, Human, Mouse, Rat, Xenopus|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide, conjugated to BSA, corresponding to the C-terminal domain of beta-tubulin isotype I.|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA1-25056 detects beta I Tubulin from human, mouse, rat, Xenopus laevis, bovine, canine, and hamster samples.
MA1-25056 has been successfully used in Western blot, ELISA, immunocytochemistry, and immunohistochemistry procedures. A suggested positive control for this product is cultured chicken fibroblasts.
The MA1-25056 immunogen is a synthetic peptide, conjugated to BSA, corresponding to the C-terminal domain of beta-tubulin isotype I.
The major building block of microtubules is tubulin. Tubulin exists as a heterodimer of two similar but not identical polypeptides, designated alpha and beta, that assembles into microtubules. For beta-tubulin, six evolutionarily conserved isotypes were identified (designated beta I - beta VI). The most complex pattern of isotype distribution in tissues is seen in the vertebrate beta-tubulins. In mammals and birds, beta l is constitutive and found in most tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Kinesin-1 motors can circumvent permanent roadblocks by side-shifting to neighboring protofilaments.
MA1-25056 was used in flow cytometry to investigate how motor proteins deal with obstacles
|Schneider R,Korten T,Walter WJ,Diez S||Biophysical journal (108:2249)||2015|