Immunofluorescent analysis of beta-2 Microglobulin in human-B2M-transfected canine cells using a beta-2 Microglobulin monoclonal antibody (Product # MA5-18119) (A) at a concentration of 2ug/ml, compared to an AlexaFluor 647-conjugated Mouse IgG2a Isotype Control (Product # MA5-18169). Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA prior to staining.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified human beta2-microglobulin|
|Conjugate||Alexa Fluor® 647|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||4ulx100ul whole blood or 10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with beta2-microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. It will not cross-react with mouse, rabbit, canine, chicken or bovine.
beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.