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Search Thermo Fisher Scientific
Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
2-4 µg/mL | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
10 µg/mL | - |
Immunocytochemistry (ICC/IF) |
Assay-dependent | - |
Flow Cytometry (Flow) |
1 µg/mL | - |
ELISA (ELISA) |
Assay-dependent | - |
Immunoprecipitation (IP) |
Assay-dependent | - |
Radioimmune Assays (RIA) |
Assay-dependent | - |
Miscellaneous PubMed (Misc) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Horse |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
B2M-01 |
Immunogen |
Purified human beta-2 Microglobulin. |
Conjugate |
Unconjugated
|
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS, pH 7.4 |
Contains |
15mM sodium azide |
Storage conditions |
4° C, do not freeze |
RRID |
AB_1070702 |
This antibody reacts with beta2-microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. It will not cross-react with mouse, rabbit, canine, chicken or bovine.
Western Blot: Both reducing and non-reducing conditions. Non-reducing conditions preferred; RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10^-8 mol/L as determined by competitive RIA.
Beta-2-microglobulin (B2M) is a 12-14 kDa protein that is a subunit of the major histocompatibility complex (MHC) class I. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells, but is absent on erythrocytes. Beta-2 microglobulin is also found in free form in the plasma. The amino acid sequence of Beta-2-microglobulin is homologous to the constant regions of immunoglobulin heavy and light chains, with a predominantly beta pleated sheet structure. The protein is ubiquitously expressed in the cytoplasm, and is involved in antigen processing and presentation. Beta-2-microglobulin also plays a role in TCR signaling, and downstream signaling in naive CD8+ cells. Beta 2-microglobulin is encoded by the B2M gene located on chromosome 15. A mutation in the Beta-2-microglobulin gene has been shown to result in hypercatabolic hypoproteinemia. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Beta-2-microglobulin that protein can be used as a prognostic marker for HIV infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-F; B2-microglobulin; Beta 2 microglobin; Beta 2 microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; Beta-2-microglobulin; Beta-2-microglobulin form pI 5.3; Beta2 microglobulin; MHC Class I; microglobulin beta-2; Orla-B2m
Gene Aliases: B2M; CDABP0092; HDCMA22P; IMD43
UniProt ID: (Human) P61769
Entrez Gene ID: (Human) 567
Molecular Function:
major histocompatibility complex protein
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