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Immunofluorescent analysis of beta-3 Tubulin (green) in SH-SY5Y cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were left untreated (left panel) or treated with 1µM staurosporine (right panel) for 3 hours and probed with a beta-3 Tubulin monoclonal antibody (Product # MA1-19187), at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan and ToxInsight at 20X magnification.
|Tested species reactivity||Many|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide|
|Purification||Ammonium sulfate precipitation, Caprylic acid precipitation|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||1:100 - 1:500|
|Immunofluorescence (IF)||1:100 - 1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||1-2 µg/ml, 90 min|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
This antibody recognizes the C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human beta III-tubulin.
For immunohistochemistry applications, this product requires protein pretreatment (0.1% trypsin) in 0.1 M HCl; incubate for 30 minutes at room temperature.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Novel crystalloid oligodendrogliopathy in hereditary spastic paraplegia.
MA1-19187 was used in immunohistochemistry to study crystalloid oligodendrogliopathy in hereditary spastic paraplegia
|Woehrer A,Laszlo L,Finsterer J,Stöllberger C,Furtner J,Rinner W,Molnar K,Budka H,Kovacs GG||Acta neuropathologica (124:583)||2012|
betaIII-tubulin; class III beta-tubulin; neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3 class III; tubulin, beta, 3; tubulin, beta, 4
3200002H15Rik; beta-4; BOS_17166; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; M(beta)3; M(beta)6; TUBB3; TUBB4