Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 6
beta-4 Tubulin Antibody (PA5-21416) in ICC/IF
Immunofluorescence analysis of beta-4 Tubulin was performed using 70 confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with beta-4 Tubulin Polyclonal Antibody (Product # PA5-21416) at 1:100 dilution in 0.1% BSA, incubated at 4 de... View More
Product Details
PA5-21416
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 238 and 410 (P04350) of beta Tubulin 4
Conjugate
Form
Liquid
Concentration
0.41 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11152108
Product Specific Information
Recommended positive controls: 293T, NIH-3T3.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (96%), Chimpanzee (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TUBB4A (beta-4 tubulin) encodes a member of the beta tubulin family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene cause hypomyelinating leukodystrophy-6 and autosomal dominant torsion dystonia-4. Alternate splicing results in multiple transcript variants encoding different isoforms. A pseudogene of this gene is found on chromosome X.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: dystonia 4, torsion (autosomal dominant); Tubulin 5 beta; tubulin beta; Tubulin beta-4 chain; Tubulin beta-4A chain; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta, 5
Gene Aliases: AI325297; beta-5; DYT4; M(beta)4; Tubb; TUBB4; TUBB4A; TUBB5
UniProt ID: (Human) P04350, (Mouse) Q9D6F9
Entrez Gene ID: (Human) 10382, (Mouse) 22153, (Rat) 29213
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support