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|Tested species reactivity||Bovine, Dog, Fruit fly, Cat, Fish, Guinea pig, Human, Mouse, Sheep, Pig, Rabbit, Rat, Xenopus|
|Published species reactivity||Bovine, Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||synthetic peptide DDDIAALVIDNGSGL conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Dot blot (DB)||Assay dependent|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunomicroscopy (IM)||Assay dependent|
|Western Blot (WB)||1:5000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
MA1-91399 detects beta Actin from Xenopus laevis, fish, Drosophila melanogaster, canine, bovine, feline, guinea pig, human, mouse, porcine, rabbit, rat and ovine samples.
MA1-91399 has been successfully used in immunocytochemistry, immunofluorescence, immunohistochemistry (frozen tissue), immunomicroscopy, Western blot, ELISA, dot blot and immunohistochemistry (paraffin tissue) applications. Positive control of cultured human or chicken fibroblasts suggested.
The MA1-91399 immunogen is synthetic peptide; DDDIAALVIDNGSGL, conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DIGE based proteome analysis of mammary gland tissue in water buffalo (Bubalus bubalis): lactating vis-a-vis heifer.
MA1-91399 was used in western blot to identify proteins which are preferentially expressed during lactationn in heifer mammary gland
|Jena MK,Janjanam J,Naru J,Kumar S,Kumar S,Singh S,Mohapatra SK,Kola S,Anand V,Jaswal S,Verma AK,Malakar D,Dang AK,Kaushik JK,Reddy VS,Mohanty AK||Journal of proteomics (119:100)||2015|
Cyclin C is a haploinsufficient tumour suppressor.
MA1-91399 was used in western blot to elucidate the regulation of Notch by Cyclin C.
|Li N,Fassl A,Chick J,Inuzuka H,Li X,Mansour MR,Liu L,Wang H,King B,Shaik S,Gutierrez A,Ordureau A,Otto T,Kreslavsky T,Baitsch L,Bury L,Meyer CA,Ke N,Mulry KA,Kluk MJ,Roy M,Kim S,Zhang X,Geng Y,Zagozdzon A,Jenkinson S,Gale RE,Linch DC,Zhao JJ,Mullighan CG,Harper JW,Aster JC,Aifantis I,von Boehmer H,Gygi SP,Wei W,Look AT,Sicinski P||Nature cell biology (16:1080)||2014|
Inhibition of intracellular hepatitis C virus replication by nelfinavir and synergistic effect with interferon-alpha.
MA1-91399 was used in western blot to study the effects of nalfinavir and IFNalpha on intracellular HCV replication
|Toma S,Yamashiro T,Arakaki S,Shiroma J,Maeshiro T,Hibiya K,Sakamoto N,Kinjo F,Tateyama M,Fujita J||Journal of viral hepatitis (16:506)||2009|
Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1.
MA1-91399 was used in western blot to investigate the important roles of Mcph1 in DNA damage-induced cellular responses
|Xu X,Lee J,Stern DF||The Journal of biological chemistry (279:34091)||2004|
actin, alpha 1, skeletal muscle; actin, beta; actin, beta, cytoplasmic; actin, cytoplasmic 1; Atcb; beta actin; beta cytoskeletal actin; beta-actin; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1; PS1TP5BP1
ACT-5; ACTA1; ACTB; Actx; beta-actin; BOS_22932; BRWS1; E430023M04Rik; PS1TP5BP1