|Tested species reactivity||Bovine, Dog, Fruit fly, Cat, Fish, Guinea pig, Human, Mouse, Sheep, Pig, Rabbit, Rat, Xenopus|
|Published species reactivity||Bovine, Zebrafish, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||synthetic peptide DDDIAALVIDNGSGL conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Dot blot (DB)||Assay dependent|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunomicroscopy (IM)||Assay dependent|
|Western Blot (WB)||1:5000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91399 detects beta Actin from Xenopus laevis, fish, Drosophila melanogaster, canine, bovine, feline, guinea pig, human, mouse, porcine, rabbit, rat and ovine samples.
MA1-91399 has been successfully used in immunocytochemistry, immunofluorescence, immunohistochemistry (frozen tissue), immunomicroscopy, Western blot, ELISA, dot blot and immunohistochemistry (paraffin tissue) applications. Positive control of cultured human or chicken fibroblasts suggested.
The MA1-91399 immunogen is synthetic peptide; DDDIAALVIDNGSGL, conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Impact of Diverse Immune Evasion Mechanisms of Cancer Cells on T Cells Engaged by EpCAM/CD3-Bispecific Antibody Construct AMG 110.
MA1-91399 was used in western blot to study the use of bispecific T cell engager
|Deisting W,Raum T,Kufer P,Baeuerle PA,Münz M||PloS one (10:null)||2015|
Deregulated Expression of SRC, LYN and CKB Kinases by DNA Methylation and Its Potential Role in Gastric Cancer Invasiveness and Metastasis.
MA1-91399 was used in western blot to assess SRC, LYN, and CKB expression, as well as their promoter methylation, in gastric cancer
|Mello AA,Leal MF,Rey JA,Pinto GR,Lamarão LM,Montenegro RC,Alves AP,Assumpção PP,Borges Bdo N,Smith MC,Burbano RR||PloS one (10:null)||2015|
Sodium fluoride affects zebrafish behaviour and alters mRNA expressions of biomarker genes in the brain: Role of Nrf2/Keap1.
MA1-91399 was used in western blot to elucidate the molecular mechanism of fluoride-induced neurotoxicity using zebrafish
|Mukhopadhyay D,Priya P,Chattopadhyay A||Environmental toxicology and pharmacology (40:352)||2015|
Effect of cellular senescence on the albumin permeability of blood-derived endothelial cells.
MA1-91399 was used in western blot to study permeability and tight junctions in endothelial cells derived from human cord blood.
|Cheung TM,Ganatra MP,Peters EB,Truskey GA||American journal of physiology. Heart and circulatory physiology (303:H1374)||2012|
DIGE based proteome analysis of mammary gland tissue in water buffalo (Bubalus bubalis): lactating vis-a-vis heifer.
MA1-91399 was used in western blot to identify proteins which are preferentially expressed during lactationn in heifer mammary gland
|Jena MK,Janjanam J,Naru J,Kumar S,Kumar S,Singh S,Mohapatra SK,Kola S,Anand V,Jaswal S,Verma AK,Malakar D,Dang AK,Kaushik JK,Reddy VS,Mohanty AK||Journal of proteomics (119:100)||2015|
Sintered indium-tin oxide particles induce pro-inflammatory responses in vitro, in part through inflammasome activation.
MA1-91399 was used in western blot to study induction of pro-inflammatory responses in vitro through sintered indium-tin oxide particles and inflammasome activation
|Badding MA,Schwegler-Berry D,Park JH,Fix NR,Cummings KJ,Leonard SS||PloS one (10:null)||2015|
Cyclin C is a haploinsufficient tumour suppressor.
MA1-91399 was used in western blot to elucidate the regulation of Notch by Cyclin C.
|Li N,Fassl A,Chick J,Inuzuka H,Li X,Mansour MR,Liu L,Wang H,King B,Shaik S,Gutierrez A,Ordureau A,Otto T,Kreslavsky T,Baitsch L,Bury L,Meyer CA,Ke N,Mulry KA,Kluk MJ,Roy M,Kim S,Zhang X,Geng Y,Zagozdzon A,Jenkinson S,Gale RE,Linch DC,Zhao JJ,Mullighan CG,Harper JW,Aster JC,Aifantis I,von Boehmer H,Gygi SP,Wei W,Look AT,Sicinski P||Nature cell biology (16:1080)||2014|
Inhibition of intracellular hepatitis C virus replication by nelfinavir and synergistic effect with interferon-alpha.
MA1-91399 was used in western blot to study the effects of nalfinavir and IFNalpha on intracellular HCV replication
|Toma S,Yamashiro T,Arakaki S,Shiroma J,Maeshiro T,Hibiya K,Sakamoto N,Kinjo F,Tateyama M,Fujita J||Journal of viral hepatitis (16:506)||2009|
Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1.
MA1-91399 was used in western blot to investigate the important roles of Mcph1 in DNA damage-induced cellular responses
|Xu X,Lee J,Stern DF||The Journal of biological chemistry (279:34091)||2004|