MA1-91399 detects beta Actin from Xenopus laevis, fish, Drosophila melanogaster, canine, bovine, feline, guinea pig, human, mouse, porcine, rabbit, rat and ovine samples.
MA1-91399 has been successfully used in immunocytochemistry, immunofluorescence, immunohistochemistry (frozen tissue), immunomicroscopy, Western blot, ELISA, dot blot and immunohistochemistry (paraffin tissue) applications. Positive control of cultured human or chicken fibroblasts suggested.
The MA1-91399 immunogen is synthetic peptide; DDDIAALVIDNGSGL, conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: actin, alpha 1, skeletal muscle; actin, beta; actin, beta, cytoplasmic; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Atcb; beta actin; beta cytoskeletal actin; Beta-actin; cytoplasmic beta-actin; cytoskeletal protein; gamma non-muscle actin; PS1TP5-binding protein 1; PS1TP5BP1
Gene Aliases: ACT-5; ACTA1; ACTB; Actx; beta-actin; BOS_22932; BRWS1; E430023M04Rik; PS1TP5BP1