Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
|Tested species reactivity||Many|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues A(26) P R A V F P S(33) of beta actin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-21167 detects beta-actin in human, mouse, rat, bovine, sheep, rabbit, feline, chicken, canine, porcine, Hirudo medicinalis (leech), Drosophila melanogaster, and guinea pig samples.
PA1-21167 has been successfully used in immunohistochemistry (FFPE) and Western blot procedures. Formalin-fixed paraffin-embedded tissue sections require boiling in 10 mM citrate buffer (pH 6.0) prior to immunostaining.
The PA1-21167 immunogen is a synthetic peptide corresponding to residues A(26) P R A V F P S(33) of beta actin.
Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Induction of hepatic apolipoprotein A-I gene expression by the isoflavones quercetin and isoquercetrin.
PA1-21167 was used in western blot to characterize the effects of quercetin, isoquercetin, and myrescetin on apo A-I gene expression in HepG2 and Caco-2 cells
|Haas MJ,Onstead-Haas LM,Szafran-Swietlik A,Kojanian H,Davis T,Armstrong P,Wong NC,Mooradian AD||Life sciences (110:8)||2014|
actin, beta; actin, beta, cytoplasmic; actin, cytoplasmic 1; Atcb; beta actin; beta cytoskeletal actin; beta-actin; cytoplasmic 1; cytoplasmic beta-actin; gamma non-muscle actin; PS1TP5-binding protein 1; PS1TP5BP1
ACT-5; ACTB; actin; Actx; Bact; beta-actin; BOS_22932; BRWS1; E430023M04Rik; PS1TP5BP1; RCJMB04_4h19